Single-cell transcriptomics analysis showing functional heterogeneity in decidual stromal cells during labor.
Single-cell transcriptomics analysis showing functional heterogeneity in decidual stromal cells during labor.
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DOI：
10.1136/jim-2020-001616
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发表时间：
2020-12-28
期刊：
影响因子：
--
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Huang J;Xie Y;Peng Q;Wang W;Pei C;Zhao Y;Liu R;Huang L;Li T;Nie J;Liu L;Zhang X;Luo X;Luo J;Zhang W
为了研究分娩过程中蜕质细胞（DSCs）的异质性及其功能改变，我们进行了单细胞RNA测序分析，以表征分娩前后蜕质细胞的转录组学特征。根据其转录组学特征，dsc（6382细胞）被聚为具有不同功能的5个亚群。与基质细胞类似，簇1中的细胞参与细胞底物粘附。另一方面，簇2和簇3中的细胞富含信号转导相关基因。分娩开始导致许多途径的显著改变，包括激活蛋白1途径（所有簇），以及对脂多糖的反应（簇1 - 3）。下调的基因参与了凝血、ATP合成和氧稳态，可能反映了分娩过程中的氧和能量平衡。我们的研究结果强调围产期dsc是异质的，在分娩中发挥多种作用。
To investigate the heterogeneity of decidual stromal cells (DSCs) and their functional alterations during delivery, we conducted single-cell RNA sequencing analysis to characterize the transcriptomic profiles of DSCs before and after labor onset. According to their transcriptomic profiles, DSCs (6382 cells) were clustered into five subgroups with different functions. Similar to stromal cells, cells in cluster 1 were involved in cell substrate adhesion. On the other hand, cells in clusters 2 and 3 were enriched in signal transduction-related genes. Labor onset led to significant alterations in many pathways, including the activator protein 1 pathway (all clusters), as well as in the response to lipopolysaccharide (clusters 1–3). The downregulated genes were involved in coagulation, ATP synthesis, and oxygen homeostasis, possibly reflecting the oxygen and energy balance during delivery. Our findings highlight that peripartum DSCs are heterogeneous and play multiple roles in labor.
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DOI：
10.1111/j.1600-0897.2010.00810.x
发表时间：
2010-06
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Thaxton JE;Sharma S
通讯作者：
Sharma S
DOI：
10.1530/rep-15-0258
发表时间：
2015-10
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Pirianov G;MacIntyre DA;Lee Y;Waddington SN;Terzidou V;Mehmet H;Bennett PR
通讯作者：
Bennett PR
影响因子：
3.6
作者：
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通讯作者：
Khan, RN
影响因子：
4.6
作者：
Silasi, Michelle;You, Yuan;Mor, Gil
通讯作者：
Mor, Gil
影响因子：
64.8
作者：
Vento-Tormo R;Efremova M;Botting RA;Turco MY;Vento-Tormo M;Meyer KB;Park JE;Stephenson E;Polański K;Goncalves A;Gardner L;Holmqvist S;Henriksson J;Zou A;Sharkey AM;Millar B;Innes B;Wood L;Wilbrey-Clark A;Payne RP;Ivarsson MA;Lisgo S;Filby A;Rowitch DH;Bulmer JN;Wright GJ;Stubbington MJT;Haniffa M;Moffett A;Teichmann SA
通讯作者：
Teichmann SA