Chemical ligation of oligodeoxynucleotides by X-irradiation and its application to regulation of G-quadruplex formation.
Chemical ligation of oligodeoxynucleotides by X-irradiation and its application to regulation of G-quadruplex formation.
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X 射线照射寡脱氧核苷酸的化学连接及其在调节 G-四链体形成中的应用。
DOI：
10.1016/j.bmcl.2013.01.123
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发表时间：
2013
影响因子：
2.7
通讯作者：
S. Nishimoto
中科院分区：
文献类型：
--
作者：
K. Tanabe;T. Mizoe;M. Sugiura;Kana Okada;S. Nishimoto
我们展示了具有二硫键的寡脱氧核苷酸（ODNs）的辐解连接及其在调控DNA四链体形成中的应用。富含G的六聚体ODNs形成四链体的能力较差，而X射线照射ODNs可诱导二硫键发生链间交换反应，形成连接的12聚体ODNs，由于熵效应，易于形成四链体。由于在K+存在下连接的ODNs与氯化血红素的络合显示出强烈的索雷特带吸收，并且还催化H2O2介导的鲁米诺氧化，因此似乎从连接的ODNs形成的四链体显示出与天然DNA四链体类似的功能。
We demonstrated radiolytic ligation of oligodeoxynucleotides (ODNs) possessing disulfide bond and its application to regulation of DNA quadruplex formation. G-rich hexamer ODNs had poor ability to form quadruplex, while X-irradiation of the ODNs induced interstrand exchange reaction at disulfide bond to form ligated 12 mer ODNs, leading to the ready formation of quadruplex due to the entropic effect. Since complexation of the ligated ODNs with hemin in the presence of K+showed strong soret band absorption and also catalyzed the H2O2-mediated oxidation of luminol, it appears that the quadruplex formed from ligated ODNs showed a function similar to native DNA quadruplex.
影响因子：
15
作者：
Kato, Y;Ohyama, T;Yamamoto, Y
通讯作者：
Yamamoto, Y
影响因子：
5.4
作者：
Matsugami, Akimasa;Xu, Yan;Katahira, Masato
通讯作者：
Katahira, Masato