Ultra-high performance liquid chromatography-size exclusion chromatography (UPLC-SEC) as an efficient tool for the rapid and highly informative characterisation of biopolymers.
Ultra-high performance liquid chromatography-size exclusion chromatography (UPLC-SEC) as an efficient tool for the rapid and highly informative characterisation of biopolymers.
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DOI：
10.1016/j.carbpol.2018.05.049
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发表时间：
2018-09-15
影响因子：
11.2
通讯作者：
Warren FJ
中科院分区：
文献类型：
--
作者：
Perez-Moral N;Plankeele JM;Domoney C;Warren FJ
UPLC-SEC用作生物聚合物表征的新工具。比现有技术更快的分析时间和更高的分辨率。高通量筛选淀粉精细结构的潜力。淀粉具有复杂的分子结构，其性质取决于其组成分子的相对链长和分支结构，其由于淀粉生物合成基因的多态性以及环境因素而变化。本文介绍了超高效体积排阻色谱在植物种子淀粉链分离中的应用。几种方法，已被用来分析淀粉中的链长分布，所有的分析时间，样品制备和分子量范围方面的限制。在这里，我们表明，链长分布可以得到显着减少的分析时间，使用超高效尺寸排阻色谱。该方法还可以显示一些精细结构特征的分辨率的改进。了解淀粉精细结构和生物合成基因之间的联系，将允许生物工程的淀粉与定制的性能。该技术可应用于对食品、饮料和制药工业有价值的一系列生物聚合物的尺寸分离和分辨率。
UPLC-SEC used as a novel tool for biopolymer characterisation. Faster analysis time and higher resolution than existing techniques. Potential for high throughput screening of starch fine structure. Starch has a complex molecular structure, with properties dependent on the relative chain lengths and branching structure of its constituent molecules, which varies due to polymorphisms in starch biosynthetic genes, as well as environmental factors. Here we present the application of ultra-high performance size exclusion chromatography to the separation of starch chains from plant seeds. Several methods, have been used to analyse chain length distributions in starch, all with limitations in terms of analysis time, sample preparation and molecular weight range. Here we demonstrate that chain length distributions can be obtained with dramatically reduced analysis time using ultra-high performance size exclusion chromatography. The method may also show improvements in resolution of some fine structural features. Understanding links between starch fine structure and biosynthetic genes will allow bioengineering of starches with tailored properties. This technique may have application to the size separation and resolution of a range of biopolymers of value to the food, drink and pharmaceutical industries.
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影响因子：
3.7
作者：
Wu AC;Morell MK;Gilbert RG
通讯作者：
Gilbert RG
影响因子：
7.4
作者：
MacNair, JE;Lewis, KC;Jorgenson, JW
通讯作者：
Jorgenson, JW
DOI：
10.1081/jlc-200053046
发表时间：
2005-01-01
影响因子：
1.3
作者：
Swartz, ME
通讯作者：
Swartz, ME
DOI：
10.1016/0141-8130(87)90034-1
发表时间：
1987-04-01
影响因子：
8.2
作者：
PFANNEMULLER, B
通讯作者：
PFANNEMULLER, B
影响因子：
11.2
作者：
Wu, Alex Chi;Li, EnPeng;Gilbert, Robert G.
通讯作者：
Gilbert, Robert G.