Integrated Functional-Omics Analysis of Thermomyces lanuginosus Reveals its Potential for Simultaneous Production of Xylanase and Substituted Xylooligosaccharides
Integrated Functional-Omics Analysis of Thermomyces lanuginosus Reveals its Potential for Simultaneous Production of Xylanase and Substituted Xylooligosaccharides
复制标题
疏棉状嗜热霉菌的综合功能组学分析揭示了其同时生产木聚糖酶和取代的低聚木糖的潜力
DOI：
10.1007/s12010-018-2873-5
复制
发表时间：
2018-09
影响因子：
3
通讯作者：
Wang Lushan
中科院分区：
文献类型：
--
作者：
Shi Zelu;Gong Weili;Zhang Lili;Dai Lin;Chen Guanjun;Wang Lushan
Thermophiles have several beneficial properties for the conversion of biomass at high temperatures. Thermomyces lanuginosus is a thermophilic filamentous fungus that was shown to secrete 40 glycoside hydrolases and 25 proteases when grown on different carbon sources. Among the 13 identified glycoside hydrolases with high expression levels, 9 were reduced sugar glycosidases (RSGs) belonging to seven GH families, and 7 of the 10 identified proteases were exopeptidases belonging to six different protease families. High expression of RSGs and exopeptidases may allow the fungus to efficiently degrade oligosaccharides and oligopeptides in saprophytic habitats. There were no xylan side chain-degrading enzymes predicted in the genome of T. lanuginosus, and only one thermophilic GH11 xylanase (g4601.t1) and one GH43 xylosidase (g3706.t1) were detected by liquid chromatography-mass spectrometry/mass spectrometry when T. lanuginosus grown on xylan, which led to the accumulation of substituted xylooligosaccharides (SXOS) during corncob xylan degradation where SXOS output made up more than 8% of the total xylan. The SXOS are beneficial prebiotics and important inducers for enzymes secretion of microorganisms. Thus, T. lanuginosus exhibits distinct advantages in utilizing cheap raw materials producing one thermostable xylanase and the high value-added SXOS as well as microbial inoculants to compost by batch fermentation.
登录
查看更多内容
影响因子：
2.9
作者：
Muraro A;Hoekstra MO;Meijer Y;Lifschitz C;Wampler JL;Harris C;Scalabrin DM
通讯作者：
Scalabrin DM
DOI：
10.1016/j.fgb.2014.04.006
发表时间：
2014-11
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
van Munster JM;Daly P;Delmas S;Pullan ST;Blythe MJ;Malla S;Kokolski M;Noltorp ECM;Wennberg K;Fetherston R;Beniston R;Yu X;Dupree P;Archer DB
通讯作者：
Archer DB
影响因子：
2.9
作者：
Gong, Weili;Zhang, Huaiqiang;Wang, Lushan
通讯作者：
Wang, Lushan
蛋白质组分析前样品制备的蛋白质沉淀方法的比较。
影响因子：
4.1
作者：
Jiang, L;He, L;Fountoulakis, M
通讯作者：
Fountoulakis, M
影响因子：
2.9
作者：
Xiaomei Zhang;Ning Liu;Fan Yang;Jinghua Li;Lushan Wang;Guanjun Chen;P. Gao
通讯作者：
Xiaomei Zhang;Ning Liu;Fan Yang;Jinghua Li;Lushan Wang;Guanjun Chen;P. Gao