Chip-mediated partnerships of the homeodomain proteins Bar and Aristaless with the LIM-HOM proteins Apterous and Lim1 regulate distal leg development
Chip-mediated partnerships of the homeodomain proteins Bar and Aristaless with the LIM-HOM proteins Apterous and Lim1 regulate distal leg development
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同源结构域蛋白 Bar 和 Aristaless 与 LIM-HOM 蛋白 Apterous 和 Lim1 的芯片介导伙伴关系调节远端腿部发育
作者：
José I. Pueyo;J. Couso
果蝇的近远端模式需要将发育中的腿分成越来越小的、离散的基因功能域。 LIM-HOM 转录因子 apterous (ap) 和 Lim1（也称为 dlim1）以及同源盒基因 Bar 和 aristaless (al) 是特定腿节发育所需基因组的一部分。我们的遗传学结果表明，第四跗节中的 Ap、Bar 和 LIM 结构域结合蛋白 Chip 之间以及前跗节中的 Al、Lim1 和 Chip 之间存在翻译后相互作用，并且这些相互作用取决于此类蛋白质的平衡量的存在。我们还观察了 Bar 和 Chip、Bar 和 Ap、Lim1 和 Chip、Al 和 Chip 之间的体外蛋白质结合。与之前 Ap 和 Chip 之间相互作用的证据一起，这些结果表明这些转录因子在腿部发育过程中形成蛋白质复合物。我们认为 LIM-HOM 功能的不同发育结果是由于给定细胞中存在的相互作用伙伴的精确身份和剂量造成的。
Proximodistal patterning in Drosophila requires division of the developing leg into increasingly smaller, discrete domains of gene function. The LIM-HOM transcription factors apterous (ap) and Lim1 (also known as dlim1), and the homeobox genes Bar and aristaless (al) are part of the gene battery required for the development of specific leg segments. Our genetic results show that there are posttranslational interactions between Ap, Bar and the LIM-domain binding protein Chip in tarsus four, and between Al, Lim1 and Chip in the pretarsus, and that these interactions depend on the presence of balanced amounts of such proteins. We also observe in vitro protein binding between Bar and Chip, Bar and Ap, Lim1 and Chip, and Al and Chip. Together with the previous evidence for interactions between Ap and Chip, these results suggest that these transcription factors form protein complexes during leg development. We propose that the different developmental outcomes of LIM-HOM function are due to the precise identity and dosage of the interacting partners present in a given cell.
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影响因子：
4.6
作者：
D. J. V. Meyel;David D O'Keefe;Stefan Thor;L. Jurata;Gordon N. Gill;John B. Thomas
通讯作者：
D. J. V. Meyel;David D O'Keefe;Stefan Thor;L. Jurata;Gordon N. Gill;John B. Thomas
DOI：
10.1073/pnas.96.5.2165
发表时间：
1999-03
影响因子：
11.1
作者：
D. Rincon-Limas;C. Lu;I. Canal;M. Calleja;C. Rodríguez-Esteban;J. Izpisúa-Belmonte;J. Botas
通讯作者：
D. Rincon-Limas;C. Lu;I. Canal;M. Calleja;C. Rodríguez-Esteban;J. Izpisúa-Belmonte;J. Botas
影响因子：
3.9
作者：
Barsotti, Elizabeth;Tan, Sugata P.;Chen, Jin-Hong
通讯作者：
Chen, Jin-Hong
影响因子：
--
作者：
L. Jurata;G. Gill
通讯作者：
L. Jurata;G. Gill
影响因子：
2.7
作者：
Rauskolb, C;Irvine, KD
通讯作者：
Irvine, KD