Chick myotendinous antigen. II. A novel extracellular glycoprotein complex consisting of large disulfide-linked subunits
Chick myotendinous antigen. II. A novel extracellular glycoprotein complex consisting of large disulfide-linked subunits
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鸡肌腱抗原。
DOI：
--
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发表时间：
1984
影响因子：
7.8
通讯作者：
D. Fambrough
中科院分区：
文献类型：
--
作者：
M. Chiquet;D. Fambrough
这份报告描述了一种新的细胞外基质成分“肌腱抗原”的生化特征，它出现在鸡肢体形态发生的早期，连接发育中的肌肉纤维、肌腱和骨骼(Chiquet，M.和D.Fambough，1984；J.Cell Biol.，98：1926-1936)。这种细胞外基质抗原是由成纤维细胞和肌肉培养物释放到介质中的分泌蛋白的主要成分；可溶形式在这里描述。这种形式的肌腱抗原是一个大的糖蛋白复合体，由几个二硫键连接的亚基组成(Mr约为150,000-240,000)。不同大小的抗原亚基是相关的，因为它们产生了非常相似的蛋白分解裂解模式。M1抗体可与变性亚基结合。抗原亚单位及其衍生的约80,000个胃蛋白酶抗性抗原域对细菌胶原酶具有抵抗力。尽管具有与纤维连接蛋白相似的亚基，但肌腱抗原在结构和抗原性上似乎与纤维连接蛋白或其他已知的细胞外基质成分无关。与肌肉培养相比，成纤维细胞中每个细胞核释放的M1抗原大约多7倍。在肌肉条件培养液中，肌腱抗原以非共价形式络合成可被[~3H]氨基葡萄糖和[~(35)S]硫酸盐大量标记的超高分子物质。这种材料对软骨素酶ABC敏感，因此似乎含有硫酸化的糖胺聚糖。我们推测，肌腱抗原可能与肌肉纤维表面的蛋白多糖相互作用，从而起到连接肌腱的作用。
This report describes the biochemical characterization of a novel extracellular matrix component, " myotendinous antigen," which appears early in chick limb morphogenesis at sites connecting developing muscle fibers, tendons, and bone ( Chiquet , M., and D. Fambrough , 1984; J. Cell Biol., 98:1926-1936). This extracellular matrix antigen is a major component of the secretory proteins released into the medium by fibroblast and muscle cultures; the soluble form is characterized here. This form of myotendinous antigen is a large glycoprotein complex consisting of several disulfide linked subunits (Mr approximately 150,000-240,000). The differently sized antigen subunits are related, since they yielded very similar proteolytic cleavage patterns. M1 antibody can bind to the denatured subunits. The antigen subunits, as well as a Mr approximately 80,000 pepsin-resistant antigenic domain derived from them, are resistant to bacterial collagenase. Despite possessing subunits similar in size to fibronectin, myotendinous antigen appears to be both structurally and antigenically unrelated to fibronectin or to other known extracellular matrix components. About seven times more M1 antigen per cell nucleus was released into the medium in fibroblast as compared to muscle cultures. In muscle conditioned medium, myotendinous antigen is noncovalently complexed to very high molecular weight material that could be heavily labeled by [3H]glucosamine and [35S]sulfate. This material is sensitive to chondroitinase ABC and hence appears to contain sulfated glycosaminoglycans. We speculate that myotendinous antigen might interact with proteoglycans on the surface of muscle fibers, thereby acting as a link to tendons.
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DOI：
--
发表时间：
1981-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
通讯作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
小鼠 IV 型原胶原羧基肽的鉴定及其对基底膜原胶原组装和结构的影响。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fessler,LI;Fessler,JH
通讯作者：
Fessler,JH
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bächinger,HP;Fessler,LI;Fessler,JH
通讯作者：
Fessler,JH
雏鸡血管和其他组织中 V 型前胶原 (AB) 的组装和加工。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fessler,LI;Kumamoto,CA;Meis,ME;Fessler,JH
通讯作者：
Fessler,JH