Promoted megakaryocytic differentiation of K562 cells through oxidative stress caused by near ultraviolet irradiation.
Promoted megakaryocytic differentiation of K562 cells through oxidative stress caused by near ultraviolet irradiation.
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DOI：
10.2478/s11658-014-0215-3
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发表时间：
2014-12
影响因子：
8.3
通讯作者：
Taya M
中科院分区：
文献类型：
--
作者：
Nurhayati RW;Ojima Y;Nomura N;Taya M
活性氧（ROS）已被证明是各种细胞活动（包括细胞分化）的重要激活剂。一些报道表明，在巨核细胞（MK）谱系的细胞分化过程中ROS的必要性。在这项研究中，我们采用近紫外线（近紫外线）照射产生内源性氧化应激的MK分化过程中的K562细胞与佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导。近紫外线照射后第1天检测到细胞内ROS水平显著增加。在分化的初始阶段，G1和G2期细胞的分数得到移动使用近紫外线照射，G2期细胞的百分比增加（从31.1%上升到68.7%）。近紫外线照射诱导的p21基因的上调，这是一种细胞周期抑制剂，表明G2期细胞被阻止进行细胞分裂。结果发现，近紫外线照射可显著提高K562细胞培养后期的高倍性（8 N和16 N）细胞比例。此外，时间推移分析表明，近紫外线照射促进巨核细胞的特异性表面标记物CD 41的表达。这是第一次报道通过近紫外线照射提高氧化应激促进PMA诱导的K562细胞MK分化。
Reactive oxygen species (ROS) have been proven to be important activators for various cellular activities, including cell differentiation. Several reports showed the necessity of ROS during cell differentiation of the megakaryocytic (MK) lineage. In this study, we employed near ultraviolet (near-UV) irradiation to generate endogenous oxidative stress in an MK differentiation process of K562 cells with phorbol 12-myristate 13-acetate (PMA) induction. A significant increase in the intracellular ROS level was detected on day 1 after near-UV irradiation. In the initial stage of differentiation, a shifted fraction of G1 and G2 phase cells was obtained using near-UV irradiation, giving an increased percentage of G2 phase cells (up from 31.1 to 68.7%). The near-UV irradiation-induced upregulation of the p21 gene, which is a cell cycle inhibitor, suggested that the G2 phase cells were prevented from undergoing cell division. It was found that the percentage of high ploidy (8N and 16N) cells was enhanced significantly at the later stage of the K562 cell culture with near-UV irradiation. Moreover, time-lapse analysis showed that near-UV irradiation encouraged the expression of CD41, a specific surface marker of megakaryocytes. This is the first report that the elevated oxidative stress through the near-UV irradiation promoted the MK differentiation of PMA-induced K562 cells.
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影响因子：
2.7
作者：
TETTEROO, PAT;MASSARO, F;BORNE, AEGKV
通讯作者：
BORNE, AEGKV
影响因子：
2
作者：
Hirose K;Monzen S;Sato H;Sato M;Aoki M;Hatayama Y;Kawaguchi H;Narita Y;Takai Y;Kashiwakura I
通讯作者：
Kashiwakura I
影响因子：
12.4
作者：
Sardina, J. L.;Lopez-Ruano, G.;Hernandez-Hernandez, A.
通讯作者：
Hernandez-Hernandez, A.
影响因子：
3.7
作者：
Ojima, Yoshihiro;Duncan, Mark Thompson;Nurhayati, Retno Wahyu;Taya, Masahito;Miller, William Martin
通讯作者：
Miller, William Martin
影响因子：
6.5
作者：
Giammona, Lisa M.;Fuhrken, Peter G.;Miller, William M.
通讯作者：
Miller, William M.