Target deletion of the cytoskeleton-associated protein palladin does not impair neurite outgrowth in mice.
Target deletion of the cytoskeleton-associated protein palladin does not impair neurite outgrowth in mice.
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DOI：
10.1371/journal.pone.0006916
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发表时间：
2009-09-04
期刊：
影响因子：
3.7
通讯作者：
Wang ZG
中科院分区：
文献类型：
--
作者：
Shu RZ;Zhang F;Liu XS;Li CL;Wang L;Tai YL;Wu XL;Yang X;Liao XD;Jin Y;Gu MM;Huang L;Pang XF;Wang ZG
帕拉丁蛋白是一种肌动蛋白骨架相关蛋白，对细胞的形态发生和运动至关重要。已有研究表明，钯蛋白定位于神经元轴突生长锥，可能在轴突延伸中起重要作用。在此之前，我们培育了palladin敲除小鼠，这些小鼠在胚胎日15.5 （E15.5）之前表现出颅神经管闭合缺陷和胚胎致死。为了进一步研究palladin在发育中的神经系统中的作用，我们检测了palladin缺失小鼠胚胎的神经支配，因为在突变小鼠胚胎大脑中检测不到200kd、140kd、90-92 kd和50kd的palladin亚型。与以往的研究结果相反，我们发现在palladin缺乏的小鼠胚胎中，轴突延伸没有受到抑制。与野生型小鼠相比，钯素缺乏小鼠的皮质神经元在神经突生长方面也没有显着差异。此外，钯素缺失小鼠和野生型小鼠的神经干细胞衍生神经元的神经突生长没有差异。综上所述，这些结果表明钯金对于小鼠正常的神经突生长是必不可少的。
Palladin is an actin cytoskeleton–associated protein which is crucial for cell morphogenesis and motility. Previous studies have shown that palladin is localized to the axonal growth cone in neurons and may play an important role in axonal extension. Previously, we have generated palladin knockout mice which display cranial neural tube closure defect and embryonic lethality before embryonic day 15.5 (E15.5). To further study the role of palladin in the developing nervous system, we examined the innervation of palladin-deficient mouse embryos since the 200 kd, 140 kd, 90–92 kd and 50 kd palladin isoforms were undetectable in the mutant mouse embryo brain. Contrary to the results of previous studies, we found no inhibition of the axonal extension in palladin-deficient mouse embryos. The cortical neurons derived from palladin-deficient mice also showed no significant difference in neurite outgrowth as compared with those from wild-type mice. Moreover, no difference was found in neurite outgrowth of neural stem cell derived-neurons between palladin-deficient mice and wild-type mice. In conclusion, these results suggest that palladin is dispensable for normal neurite outgrowth in mice.
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影响因子：
20.3
作者：
Liu, Xue-Song;Li, Xi-Hua;Wang, Zhu-Gang
通讯作者：
Wang, Zhu-Gang
影响因子：
16.2
作者：
Wills, Z;Marr, L;Van Vactor, D
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Van Vactor, D
影响因子：
4
作者：
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影响因子：
3.5
作者：
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Wang, ZG
影响因子：
3.3
作者：
Boukhelifa, M;Parast, MM;Otey, CA
通讯作者：
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