Characterization of an Atypical Metalloproteinase Inhibitors Like Protein (Sbp8-1) From Scallop Byssus.
Characterization of an Atypical Metalloproteinase Inhibitors Like Protein (Sbp8-1) From Scallop Byssus.
复制标题
扇贝足丝非典型金属蛋白酶抑制剂类蛋白 (Sbp8-1) 的表征
DOI：
10.3389/fphys.2018.00597
复制
发表时间：
2018
影响因子：
4
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Zhang X;Dai X;Wang L;Miao Y;Xu P;Liang P;Dong B;Bao Z;Wang S;Lyu Q;Liu W
黏附是包括贻贝和扇贝在内的许多海洋软体动物的一个重要的生理过程，因此对这些黏附中所涉及的蛋白质进行表征是很重要的。虽然已经鉴定和鉴定了几种贻贝贝壳蛋白，但对扇贝贝壳蛋白的研究仍然很少。我们先前的研究发现了两个足部特异性蛋白(Sbp7，Sbp8-1)，它们被注释为金属蛋白酶组织抑制因子(TIMPs)。进化分析表明，栉孔扇贝TIMP基因在进化过程中经历了多个基因的复制，其在足部的潜在功能可能有一个古老的进化起源。序列比对和生化分析表明，Sbp8-1是一种非典型的TIMP。一个重要的特征是在其C末端存在两个额外的游离半胱氨酸残基，这导致Sbp8-1聚合。考虑到这种非典型的Sbp8-1可能作为交联剂在扇贝体内发挥着交联剂的作用，我们认为这种非典型的Sbp8-1可能作为交联剂在扇贝体内发挥作用。本研究不仅有助于理解扇贝的骨性组装，也为防水材料的设计提供了启示。
Adhesion is a vital physiological process for many marine molluscs, including the mussel and scallop, and therefore it is important to characterize the proteins involved in these adhesives. Although several mussel byssal proteins were identified and characterized, the study for scallop byssal proteins remains scarce. Our previous study identified two foot-specific proteins (Sbp7, Sbp8-1), which were annotated as the tissue inhibitors of metalloproteinases (TIMPs). Evolutionary analysis suggests that the TIMP genes of Chlamys farreri had gone through multiple gene duplications during evolution, and their potential functional roles in foot may have an ancient evolutionary origin. Focusing on the Sbp8-1, the sequence alignment and biochemical analyses suggest that Sbp8-1 is an atypical TIMP. One significant feature is the presence of two extra free Cys residues at its C-terminus, which causes the Sbp8-1 polymerization. Considering the fact that the no inhibitory activity was observed and it is mainly distributed in byssal thread and plaque, we proposed that this atypical Sbp8-1 may play as the cross-linker in scallop byssus. This study facilitates not only the understanding of scallop byssus assembly, also provides the inspiration of water-resistant materials design.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
DOI：
10.1007/978-1-4614-0631-0_34
发表时间：
2012-01-01
期刊：
RETINAL DEGENERATIVE DISEASES
影响因子：
--
作者：
Stoehr, Heidi;Anand-Apte, Bela
通讯作者：
Anand-Apte, Bela
影响因子：
9.7
作者：
Lee BP;Messersmith PB;Israelachvili JN;Waite JH
通讯作者：
Waite JH
影响因子：
2.9
作者：
Rao, SK;Mathrubutham, M;Cohen, JR
通讯作者：
Cohen, JR
影响因子：
6.2
作者：
Sun, CJ;Lucas, JM;Waite, JH
通讯作者：
Waite, JH