Age and Sex-Related Changes to Gene Expression in the Mouse Spinal Cord
Age and Sex-Related Changes to Gene Expression in the Mouse Spinal Cord
复制标题
小鼠脊髓基因表达与年龄和性别相关的变化
DOI：
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发表时间：
2019
影响因子：
3.1
通讯作者：
M. Wilson
中科院分区：
文献类型：
--
作者：
Jeremy McCallum;Greg Anderson;M. Wilson
脊髓是大脑和身体其他部分之间神经元通讯的关键。为了进一步了解支持小鼠脊髓成熟的分子变化，我们分析了4周龄（青春期开始前）和成年期（8周）之间的基因表达差异。我们发现800个基因在幼年和成年脊髓中有显著差异表达。基因本体分析显示，在髓鞘形成和信号转导等作用的基因的过度表达。另外19个基因的表达是性二态性的，包括常染色体和性连锁基因。鉴于脊髓中存在类固醇激素受体，我们还研究了两种主要类固醇激素雌二醇和双氢睾酮（DHT）对脊髓基因表达的影响。在性腺切除的雄性动物中，雌二醇和DHT的植入物对脊髓基因表达产生了显着的变化。这项研究概述了在脊髓成熟的关键时期，随着脊髓成熟而发生的全球基因表达变化。这证实了在涉及脊髓的研究中，年龄和性别都是重要的考虑因素。
The spinal cord is essential for neuronal communication between the brain and rest of the body. To gain further insight into the molecular changes underpinning maturation of the mouse spinal cord, we analysed gene expression differences between 4 weeks of age (prior to puberty onset) and adulthood (8 weeks). We found 800 genes were significantly differentially expressed between juvenile and adult spinal cords. Gene ontology analysis revealed an overrepresentation of genes with roles in myelination and signal transduction among others. The expression of a further 19 genes was sexually dimorphic; these included both autosomal and sex-linked genes. Given the presence of steroid hormone receptors in the spinal cord, we also looked at the impact of two major steroid hormones, oestradiol and dihydrotestosterone (DHT) on spinal cord gene expression for selected genes. In gonadectomised male animals, implants with oestradiol and DHT produced significant changes to spinal cord gene expression. This study provides an overview of the global gene expression changes that occur as the spinal cord matures, over a key period of maturation. This confirms that both age and sex are important considerations in studies involving the spinal cord.
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影响因子：
7
作者：
Yang, Fan;Babak, Tomas;Disteche, Christine M.
通讯作者：
Disteche, Christine M.
影响因子：
4.8
作者：
Salih, DAM;Mohan, S;Pell, JM
通讯作者：
Pell, JM
DOI：
10.1146/annurev-cellbio-100913-013038
发表时间：
2015
影响因子：
11.3
作者：
Riccomagno MM;Kolodkin AL
通讯作者：
Kolodkin AL
DOI：
10.1530/joe-15-0360
发表时间：
2016-03
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Corre C;Shinoda G;Zhu H;Cousminer DL;Crossman C;Bellissimo C;Goldenberg A;Daley GQ;Palmert MR
通讯作者：
Palmert MR
影响因子：
44.5
作者：
Abreu, Ana Paula;Kaiser, Ursula B.
通讯作者：
Kaiser, Ursula B.