Modulation of eDNA release and degradation affects Staphylococcus aureus biofilm maturation.
Modulation of eDNA release and degradation affects Staphylococcus aureus biofilm maturation.
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EDNA释放和降解的调节会影响金黄色葡萄球菌生物膜成熟。
DOI：
10.1371/journal.pone.0005822
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发表时间：
2009-06-09
期刊：
影响因子：
3.7
通讯作者：
Bayles KW
中科院分区：
文献类型：
--
作者：
Mann EE;Rice KC;Boles BR;Endres JL;Ranjit D;Chandramohan L;Tsang LH;Smeltzer MS;Horswill AR;Bayles KW
最近的研究表明，金黄色葡萄球菌介导的细胞裂解和基因组DNA释放在生物膜粘附中的作用。目前的研究通过检查生物膜成熟过程中基因组DNA释放和降解控制中涉及的时间和其他遗传因素来扩展这些发现。细胞裂解和DNA释放被认为是至关重要的生物膜附着在发育的初始阶段和释放的DNA（eDNA）仍然是一个重要的基质成分在生物膜成熟。该研究还揭示了lrgAB突变体表现出增加的生物膜粘附和基质相关的eDNA，这与其作为IgA介导的裂解的抑制剂的所提出的作用一致。在流动细胞分析中，cid和lrg突变对生物膜成熟和塔形成有显著影响。最后，葡萄球菌热核酸酶被证明参与生物膜的发展，作为一个nuc突变体形成了一个更厚的生物膜含有增加水平的基质相关的eDNA。总之，这些研究结果表明，在生物膜发育过程中，cid和lrg基因产物的相反活性控制细胞裂解和基因组DNA释放，而葡萄球菌热核酸酶的功能是降解eDNA，可能是促进生物膜分散的一种手段。
Recent studies have demonstrated a role for Staphylococcus aureus cidA-mediated cell lysis and genomic DNA release in biofilm adherence. The current study extends these findings by examining both temporal and additional genetic factors involved in the control of genomic DNA release and degradation during biofilm maturation. Cell lysis and DNA release were found to be critical for biofilm attachment during the initial stages of development and the released DNA (eDNA) remained an important matrix component during biofilm maturation. This study also revealed that an lrgAB mutant exhibits increased biofilm adherence and matrix-associated eDNA consistent with its proposed role as an inhibitor of cidA-mediated lysis. In flow-cell assays, both cid and lrg mutations had dramatic effects on biofilm maturation and tower formation. Finally, staphylococcal thermonuclease was shown to be involved in biofilm development as a nuc mutant formed a thicker biofilm containing increased levels of matrix-associated eDNA. Together, these findings suggest a model in which the opposing activities of the cid and lrg gene products control cell lysis and genomic DNA release during biofilm development, while staphylococcal thermonuclease functions to degrade the eDNA, possibly as a means to promote biofilm dispersal.
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影响因子：
3.6
作者：
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通讯作者：
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