Clinical significance of p16 protein expression loss and aberrant p53 protein expression in pancreatic cancer.
Clinical significance of p16 protein expression loss and aberrant p53 protein expression in pancreatic cancer.
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DOI：
10.3349/ymj.2005.46.4.519
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发表时间：
2005-08-31
影响因子：
2.4
通讯作者：
Kim BR
中科院分区：
文献类型：
--
作者：
Jeong J;Park YN;Park JS;Yoon DS;Chi HS;Kim BR
胰腺癌是一种预后不良的疾病，主要是由于切除率低和诊断晚。为了提高可切除性和提高生存率，需要更好地了解胰腺癌发病机制和更有效的筛查技术。新方法，例如遗传和分子改变，可能会提出胰腺癌诊断和治疗的新方法。我们使用单克隆抗 p16 抗体和单克隆抗 p53 抗体对 44 份福尔马林固定、石蜡包埋的胰腺导管腺癌标本进行了免疫组织化学研究。使用SPSS卡方检验比较p16和p53蛋白的表达。使用 Kaplan-Meier 方法分析无病生存率，并通过对数秩检验进行验证。 20 例 (45.5%) 病例发现 p16 表达缺失，14 例 (31.8%) 病例检测到 p53 蛋白表达异常。尽管 p16 表达与生存之间没有显着相关性，但 p16 表达缺失与淋巴结转移发生率较高 (p=0.040) 和晚期分期 (p=0.015) 相关。异常的 p53 蛋白表达与组织学分级相关 (p=0.038)。与阴性组相比，异常p53蛋白阳性组的无病生存率显着较低（p=0.029）。根据我们的结果，我们认为 p53 不是一个预后因素；然而，p16 和 p53 基因确实在胰腺导管腺癌的进展中发挥重要作用。
Pancreatic cancer is a disease with poor prognosis mainly due to low resection rates and late diagnosis. To increase resectability and improve survival rates, a better understanding of pancreatic cancer pathogenesis and more effective screening techniques are required. New methods, such as genetic and molecular alterations, may suggest novel approaches for pancreatic cancer diagnosis and treatment. We immunohistochemically investigated 44 formalin-fixed, paraffin-embedded specimens of pancreatic ductal adenocarcinoma using monoclonal anti-p16 antibodies and monoclonal anti-p53 antibodies. The expressions of p16 and p53 proteins were compared using the Chi-square test with SPSS. Disease-free survival was analyzed using the Kaplan-Meier method, verified by the Log-Rank test. Loss of p16 expression was noted in 20 (45.5%) cases and aberrant p53 protein expression was detected in 14 (31.8%) cases. Loss of p16 expression was associated with a higher incidence of lymph node metastasis (p=0.040) and a more advanced stage (p=0.015), although there was no significant correlation between p16 expression and survival. Aberrant p53 protein expression correlated with histologic grade (p=0.038). Disease-free survival rate was significantly lower in the aberrant p53 protein positive group compared to the negative group (p=0.029). From our results, we suggest that p53 is not a prognostic factor; however, p16 and p53 genes do play important roles in the progression of pancreatic ductal adenocarcinoma.
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影响因子：
2.3
作者：
Gansauge, F;Gansauge, S;Beger, HG
通讯作者：
Beger, HG
影响因子：
2.5
作者：
Nio, Y;Dong, M;Tamura, K
通讯作者：
Tamura, K
影响因子：
2.9
作者：
Campani, D;Boggi, U;Fornaciari, G
通讯作者：
Fornaciari, G
影响因子：
2.4
作者：
Yoon, DS;Jeong, J;Kim, BR
通讯作者：
Kim, BR
DOI：
10.1093/jnci/88.10.680
发表时间：
1996-05-15
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Bartsch, D;Shevlin, DW;Goodfellow, PJ
通讯作者：
Goodfellow, PJ