Maternal inheritance of twist and analysis of MAPK activation in embryos of the polychaete annelid Platynereis dumerilii.
Maternal inheritance of twist and analysis of MAPK activation in embryos of the polychaete annelid Platynereis dumerilii.
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DOI：
10.1371/journal.pone.0096702
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wolfstetter G
中科院分区：
文献类型：
--
作者：
Pfeifer K;Schaub C;Domsch K;Dorresteijn A;Wolfstetter G
在这项研究中，我们的目的是确定在Platynereis dumerilii中4d （mesentoblast）谱系的分子机制。我们采用RT-PCR和对Platynereis dumerilii twist同源物（pdul -twist）的原位杂交来揭示该谱系中的中胚层规范。我们发现Pdu-twist mRNA已经在母体中分布。受精后，卵浆分离导致Pdu-twist转录本重新定位到成体细胞谱系（2d）和4d，这表明Pdu-twist的母系成分可能是进一步中胚层发育的重要先决条件，但并不代表中胚层细胞的决定性特征。然而，在原始生殖细胞从4d谱系分离后，在肌源性祖细胞中只观察到Pdu-twist的合子转录，这表明中胚层分化发生在4d阶段之后。先前对螺旋切割胚胎的研究揭示了4d谱系与能够诱导某些微粒发育命运的胚胎组织者活性之间的时空相关性。这就提出了一个问题，即四维谱系的规范是否可以与组织者的活动联系起来。因此，我们的目的是通过对dpERK的抗体染色来揭示Platynereis中这种保守组织者的存在。与之前在其他螺旋胚胎中的观察结果相反，在2d和4d形成过程中无法检测到MAPK信号的激活，这就质疑了组织者功能和4d谱系规范之间是否存在保守联系。然而，我们的实验揭示了MAPK在准肾母细胞以及原肠胚的大粒和一些微粒中的强大激活。抑制MAPK的激活会导致幼虫体轴缩短，躯干肌肉扩张缺陷和神经系统收缩不正常，这表明MAPK信号在原肠胚形成过程中对胚胎组织的重组起着关键作用。
In this study, we aimed to identify molecular mechanisms involved in the specification of the 4d (mesentoblast) lineage in Platynereis dumerilii. We employ RT-PCR and in situ hybridization against the Platynereis dumerilii twist homolog (Pdu-twist) to reveal mesodermal specification within this lineage. We show that Pdu-twist mRNA is already maternally distributed. After fertilization, ooplasmatic segregation leads to relocation of Pdu-twist transcripts into the somatoblast (2d) lineage and 4d, indicating that the maternal component of Pdu-twist might be an important prerequisite for further mesoderm specification but does not represent a defining characteristic of the mesentoblast. However, after the primordial germ cells have separated from the 4d lineage, zygotic transcription of Pdu-twist is exclusively observed in the myogenic progenitors, suggesting that mesodermal specification occurs after the 4d stage. Previous studies on spiral cleaving embryos revealed a spatio-temporal correlation between the 4d lineage and the activity of an embryonic organizer that is capable to induce the developmental fates of certain micromeres. This has raised the question if specification of the 4d lineage could be connected to the organizer activity. Therefore, we aimed to reveal the existence of such a proposed conserved organizer in Platynereis employing antibody staining against dpERK. In contrast to former observations in other spiralian embryos, activation of MAPK signaling during 2d and 4d formation cannot be detected which questions the existence of a conserved connection between organizer function and specification of the 4d lineage. However, our experiments unveil robust MAPK activation in the prospective nephroblasts as well as in the macromeres and some micromeres at the blastopore in gastrulating embryos. Inhibition of MAPK activation leads to larvae with a shortened body axis, defects in trunk muscle spreading and improper nervous system condensation, indicating a critical function for MAPK signaling for the reorganization of embryonic tissues during the gastrulation process.
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DOI：
10.1007/bf00376021
发表时间：
1987-01-01
期刊：
ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
DORRESTEIJN, AWC;BORNEWASSER, H;FISCHER, A
通讯作者：
FISCHER, A
影响因子：
2.6
作者：
Henry, Jonathan Q.;Perry, Kimberly J.;Martindale, Mark Q.
通讯作者：
Martindale, Mark Q.
影响因子：
1.5
作者：
Ackermann, C;Dorresteijn, A;Fischer, A
通讯作者：
Fischer, A
影响因子：
4.8
作者：
Favata, MF;Horiuchi, KY;Trzaskos, JM
通讯作者：
Trzaskos, JM
影响因子：
4.1
作者：
Giani VC Jr;Yamaguchi E;Boyle MJ;Seaver EC
通讯作者：
Seaver EC