Somatic and germline expression of piwi during development and regeneration in the marine polychaete annelid Capitella teleta.
Somatic and germline expression of piwi during development and regeneration in the marine polychaete annelid Capitella teleta.
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DOI：
10.1186/2041-9139-2-10
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发表时间：
2011-05-05
期刊：
影响因子：
4.1
通讯作者：
Seaver EC
中科院分区：
文献类型：
--
作者：
Giani VC Jr;Yamaguchi E;Boyle MJ;Seaver EC
干细胞在成人的生长和再生过程中起着至关重要的作用。生殖系干细胞是一种特殊的干细胞，在有性繁殖过程中产生配子。Capitella teleta(前身为Capitella sp.是一种多毛类环节动物，具有有性繁殖、成体生长和再生，因此是研究体细胞和生殖系干细胞关系的良好模型。我们表征了Piwi的两个C.teleta同源基因的表达，这两个基因在不同的生物体中起到了生殖系发育的作用。Ct-piwi1和Ct-piwi2在整个生命周期中以动态模式表达，包括体细胞和生殖细胞，并在所研究的所有阶段显示出几乎相同的表达模式。这两个基因在胚胎和幼虫发育过程中广泛表达，逐渐局限于假定的原始生殖细胞(PGCs)和后部生长区。在幼体中，CT-piwi1在推定的性腺中表达，在生殖成体中，它在性腺和后生长带中检测到。此外，Ct-piwi1在一群假定的PGC中表达，这些PGC在性成熟成人性成熟中持续存在，很可能在干细胞利基中表达。CT-piwi1在再生组织中表达，一旦分化，在再生段的后部生长带和再生卵巢中的未成熟卵母细胞中表达最明显。在C.teleta中，piwi基因可能保留了作为体细胞和生殖系干细胞的遗传调节器的祖先角色。在进化过程中，Piwi基因和相关的干细胞共同调节因子很可能局限于某些分类群的种系。
Stem cells have a critical role during adult growth and regeneration. Germline stem cells are specialized stem cells that produce gametes during sexual reproduction. Capitella teleta (formerly Capitella sp. I) is a polychaete annelid that reproduces sexually, exhibits adult growth and regeneration, and thus, is a good model to study the relationship between somatic and germline stem cells. We characterize expression of the two C. teleta orthologs of piwi, genes with roles in germline development in diverse organisms. Ct-piwi1 and Ct-piwi2 are expressed throughout the life cycle in a dynamic pattern that includes both somatic and germline cells, and show nearly identical expression patterns at all stages examined. Both genes are broadly expressed during embryonic and larval development, gradually becoming restricted to putative primordial germ cells (PGCs) and the posterior growth zone. In juveniles, Ct-piwi1 is expressed in the presumptive gonads, and in reproductive adults, it is detected in gonads and the posterior growth zone. In addition, Ct-piwi1 is expressed in a population of putative PGCs that persist in sexually mature adults, likely in a stem cell niche. Ct-piwi1 is expressed in regenerating tissue, and once segments differentiate, it becomes most prominent in the posterior growth zone and immature oocytes in regenerating ovaries of regenerating segments. In C. teleta, piwi genes may have retained an ancestral role as genetic regulators of both somatic and germline stem cells. It is likely that piwi genes, and associated stem cell co-regulators, became restricted to the germline in some taxa during the course of evolution.
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影响因子：
2.4
作者：
ECKELBARGER, KJ;GRASSLE, JP
通讯作者：
GRASSLE, JP
影响因子：
2.7
作者：
Alie, Alexandre;Leclere, Lucas;Manuel, Michael
通讯作者：
Manuel, Michael
影响因子：
0.7
作者：
Molyneaux, K;Wylie, C
通讯作者：
Wylie, C
影响因子：
4
作者：
Fischer, A;Dorresteijn, A
通讯作者：
Dorresteijn, A
影响因子：
56.9
作者：
GRASSLE, JP;GRASSLE, JF
通讯作者：
GRASSLE, JF