Electrophoretic mobility of linear and star‐branched DNA in semidilute polymer solutions
Electrophoretic mobility of linear and star‐branched DNA in semidilute polymer solutions
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线性和星形支化 DNA 在半稀聚合物溶液中的电泳迁移率
DOI：
10.1002/elps.200500836
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发表时间：
2006
期刊：
影响因子：
2.9
通讯作者：
L. Archer
中科院分区：
文献类型：
--
作者：
S. Saha;Daniel M. Heuer;L. Archer
用荧光可视化技术研究了线形T2(162kBP)和3臂星形分支(NARM = 48.5kBP)DNA在高于重叠浓度c*的线型聚丙烯酰胺溶液中的电泳性，该技术可以确定μ的构象和迁移率。筛分介质为中等多分散指数(PI MDA1.7~2.1)和可变聚合物相对分子质量(0.5 9~2.0 5 ∼ )的LPA溶液。在非纠缠半稀溶液(c*Ce)中，迁移的线状和星形DNA的构象与其非纠缠溶液的性质仅有细微的变化，但恒星的μ随着LPA浓度和相对分子质量的增加而急剧下降，而线形DNA的μ几乎与c和MW无关。这些发现是在目前大分子聚电解质的电泳学理论的背景下讨论的。
Electrophoresis of large linear T2 (162 kbp) and 3‐arm star‐branched (NArm = 48.5 kbp) DNA in linear polyacrylamide (LPA) solutions above the overlap concentration c* has been investigated using a fluorescence visualization technique that allows both the conformation and mobility μ of the DNA to be determined. LPA solutions of moderate polydispersity index (PI ∼ 1.7–2.1) and variable polymer molecular weight Mw (0.59–2.05 MDa) are used as the sieving media. In unentangled semidilute solutions (c*ce), the conformation of migrating linear and star‐shaped DNA manifest only subtle changes from their unentangled solution features, but μ for the stars decreases strongly with increasing LPA concentration and molecular weight, while μ for linear DNA becomes nearly independent of c and Mw. These findings are discussed in the context of current theories for electrophoresis of large polyelectrolytes.
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影响因子：
14.9
作者：
RYE, HS;YUE, S;GLAZER, AN
通讯作者：
GLAZER, AN
聚合物溶液中高于和低于缠结极限的 DNA 构象动力学。
影响因子：
7.4
作者：
Shi,X;Hammond,RW;Morris,MD
通讯作者：
Morris,MD
影响因子：
2.9
作者：
Lu, YJ;Weers, B;Stellwagen, NC
通讯作者：
Stellwagen, NC
影响因子：
7.4
作者：
Woolley, AT;Lao, KQ;Mathies, RA
通讯作者：
Mathies, RA