Intestinal epithelial stem cells do not protect their genome by asymmetric chromosome segregation.
Intestinal epithelial stem cells do not protect their genome by asymmetric chromosome segregation.
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DOI：
10.1038/ncomms1260
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发表时间：
2011-03-29
影响因子：
16.6
通讯作者：
Legraverend, Catherine
中科院分区：
文献类型：
--
作者：
Escobar, Marion;Nicolas, Pierre;Sangar, Fatiha;Laurent-Chabalier, Sabine;Clair, Philippe;Joubert, Dominique;Jay, Philippe;Legraverend, Catherine
具有高周转率的成体组织的干细胞通过在连续分裂中保持相同的“不朽”模板DNA链而免受DNA复制诱导的突变的影响，这一想法已经在几种组织中进行了测试。在小肠上皮中，所提供的证据是基于干细胞位于潘氏细胞上方的假设。遗传谱系追踪实验的结果指出，潘氏细胞之间的隐窝基底柱状细胞是真正的干细胞。在这里，我们表明，这些细胞分离的大部分，如果不是全部，他们的染色体随机，在完整的和再生上皮。因此，“不朽”的模板DNA链假设不适用于肠上皮干细胞，肠上皮干细胞必须依靠其他策略来避免累积突变。 有人提出，干细胞使用非随机染色体分离，以避免复制诱导突变的积累。在这里，作者研究了肠上皮干细胞分裂，并使用标记排除和保留试验表明，细胞随机分离染色体。
The idea that stem cells of adult tissues with high turnover are protected from DNA replication-induced mutations by maintaining the same 'immortal' template DNA strands together through successive divisions has been tested in several tissues. In the epithelium of the small intestine, the provided evidence was based on the assumption that stem cells are located above Paneth cells. The results of genetic lineage-tracing experiments point instead to crypt base columnar cells intercalated between Paneth cells as bona fide stem cells. Here we show that these cells segregate most, if not all, of their chromosomes randomly, both in the intact and in the regenerating epithelium. Therefore, the 'immortal' template DNA strand hypothesis does not apply to intestinal epithelial stem cells, which must rely on other strategies to avoid accumulating mutations. It has been proposed that stem cells use nonrandom chromosome segregation to avoid the accumulation of replication-induced mutations. Here, the authors examine intestinal epithelial stem-cell division and show, using label exclusion and retention assays, that the cells segregate their chromosomes randomly.
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影响因子：
30.8
作者：
Sangiorgi, Eugenio;Capecchi, Mario R.
通讯作者：
Capecchi, Mario R.
影响因子：
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作者：
通讯作者：
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DOI：
10.1152/ajpgi.00160.2005
发表时间：
2005-09-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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