Circulating Fatty Objects and Their Preferential Presence in Pancreatic Cancer Patient Blood Samples.
Circulating Fatty Objects and Their Preferential Presence in Pancreatic Cancer Patient Blood Samples.
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DOI：
10.3389/fphys.2022.827531
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发表时间：
2022
影响因子：
4
通讯作者：
Pandol SJ
中科院分区：
文献类型：
--
作者：
Wang R;Nissen NN;Zhang Y;Shao C;Chu CY;Huynh C;Posadas EM;Tomlinson JS;Lewis MS;Pandol SJ
人类癌症通常并发血管闭塞的发生率增加，而血管闭塞大多对抗凝治疗不敏感。我们寻找癌症相关栓塞的因果因素。共检查了2，017份血液样本的可见异常。检查的是来自癌症患者的外周血样本，这些患者即将接受泌尿生殖系统、乳腺、胃肠道或腹部肿瘤的手术治疗。研究纳入了来自接受复发性或去势抵抗性前列腺癌治疗的非卧床患者的样本。用亲脂性染色和脂质组分析研究了富脂性，而用特异性染色和抗体检测评估了表面膜。我们发现了一种新的实体，脂滴样物体或循环脂肪物体（CFO），在许多癌症患者的血液样本中可见，具有引起栓塞的潜力。CFO被定义为具有膜的富含脂质的物体，能够通过与离体培养中的外周血单核细胞相互作用而增加体积。来自胰腺癌患者的血液样本被发现具有最高的CFO发生率和最大的CFO数量。最值得注意的是，来自许多胰腺癌样本的CFO呈现为缠绕在不溶性纤维网络中的大簇，这表明血管内凝血。本研究将CFO确定为癌症患者血液中的异常实体，并且是癌症发展和进展期间血管内栓塞的促成因素。
Human cancers are often complicated with increased incidences of blood vessel occlusion, which are mostly insensitive to anticoagulation therapy. We searched for causal factors of cancer-associated embolism. A total of 2,017 blood samples was examined for visible abnormalities. Examined were peripheral blood samples from cancer patients who were about to undergo surgical treatment for genitourinary, breast, gastrointestinal or abdominal tumors. Samples from ambulatory patients being treated for recurrent or castration-resistant prostate cancers were included in the study. The lipid-rich nature was studied with lipophilic stains and lipid panel analysis, while surface membrane was assessed with specific staining and antibody detection. We identified a new entity, lipid droplet-like objects or circulating fatty objects (CFOs), visible in the blood samples of many cancer patients, with the potential of causing embolism. CFOs were defined as lipid-rich objects with a membrane, capable of gaining in volume through interaction with peripheral blood mononuclear cells in ex vivo culture. Blood samples from pancreatic cancer patients were found to have the highest CFO incidence and largest CFO numbers. Most noticeably, CFOs from many pancreatic cancer samples presented as large clusters entangled in insoluble fiber networks, suggestive of intravascular clotting. This study identifies CFO as an abnormal entity in cancer patient blood, and a contributory factor to intravascular embolism during cancer development and progression.
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影响因子：
3.7
作者：
Norouzi N;Bhakta HC;Grover WH
通讯作者：
Grover WH
DOI：
10.4137/cmo.s18991
发表时间：
2014
期刊：
Clinical Medicine Insights. Oncology
影响因子：
--
作者：
Elyamany G;Alzahrani AM;Bukhary E
通讯作者：
Bukhary E
影响因子：
11.5
作者：
Khorana, Alok A.;Ahrendt, Steven A.;Taubman, Mark B.
通讯作者：
Taubman, Mark B.
影响因子：
24.5
作者：
Portincasa, P;VanErpecum, KJ;vanBergeHenegouwen, GP
通讯作者：
vanBergeHenegouwen, GP
影响因子：
15.9
作者：
ADMIRAND, WH;SMALL, DM
通讯作者：
SMALL, DM