Suitable Materials for Soft Tissue Reconstruction: In Vitro Studies of Cell – Triblock Copolymer Interactions
Suitable Materials for Soft Tissue Reconstruction: In Vitro Studies of Cell – Triblock Copolymer Interactions
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适用于软组织重建的材料：细胞-三嵌段共聚物相互作用的体外研究
DOI：
10.1177/0883911505058608
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发表时间：
2005
影响因子：
1.7
通讯作者：
N. Andronova
中科院分区：
文献类型：
--
作者：
M. Mattioli;G. Biagini;G. Lucarini;L. Virgili;F. Gabbanelli;S. Amati;Francesca Cecchet;A. Albertsson;A. Finne;N. Andronova
角质形成细胞和成纤维细胞已经生长在一系列基于1，5-二氧杂环庚烷-2-酮（DXO）和L-丙交酯（LLA）的三嵌段共聚物上。共聚物中DXO和LLA的摩尔比是变化的。这导致不同程度的亲水性，这反过来又影响细胞生长。在这些表面上，通过扫描电子显微镜，延时视频显微镜和免疫组织化学的细胞的运动和生长的细胞的形态外观进行了研究。所有结果清楚地表明，角质形成细胞和成纤维细胞最好地粘附于最亲水的共聚物。接种在最亲水的共聚物上的大多数角质形成细胞也呈现出极化形态，表明迁移趋势。细胞在这些材料上的生长是令人感兴趣的，因为这些独特材料的可能应用是作为用于引导皮肤和/或牙周组织生成的聚合物膜。
Keratinocytes and fibroblasts have been grown onto a series of triblock copolymers based on 1,5-dioxepan-2-one (DXO) and L-lactide (LLA). The molar ratio of DXO and LLA were varied in the copolymers. This resulted in different degrees of hydrophilicity, which in turn influenced the cell growth. On these surfaces, the morphological appearance of the cells with their cell movements and growth were investigated by means of scanning electron microscopy, time-lapse videomicroscopy and immunohistochemistry. All results clearly showed that the keratinocytes and fibroblasts adhered best to the most hydrophilic copolymers. A majority of the keratinocytes seeded on the most hydrophilic copolymer also presented a polarized morphology indicating a migration tendency. The cell growth onto these materials are interesting since a possible application for these unique materials is as polymeric membranes for guided cutaneous and/or periodontal tissue generation.
DOI：
10.1002/(sici)1097-4636(19980905)41:3
发表时间：
1998-09-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Webb, K;Hlady, V;Tresco, PA
通讯作者：
Tresco, PA