High-speed two-photon excited autofluorescence imaging of ex vivo human retinal pigment epithelial cells toward age-related macular degeneration diagnostic.
High-speed two-photon excited autofluorescence imaging of ex vivo human retinal pigment epithelial cells toward age-related macular degeneration diagnostic.
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离体人视网膜色素上皮细胞的高速双光子激发自发荧光成像用于年龄相关性黄斑变性的诊断。
DOI：
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发表时间：
2008
影响因子：
3.5
通讯作者：
J. Bille
中科院分区：
文献类型：
--
作者：
Olivier La Schiazza;J. Bille
老年性黄斑变性(AMD)是眼科关注的主要问题之一，在发达国家是导致不可逆性失明的主要原因。然而，人们对这种重要的眼部疾病的起源和触发机制知之甚少。在常见的临床实践中，AMD是通过共焦扫描激光检眼镜对视网膜色素上皮(RPE)细胞进行自体荧光成像来监测的。RPE细胞的主要自发荧光来源于随着年龄和疾病的增加而积聚在细胞质中的脂褐素颗粒。我们探索了一种使用双光子激发的自体荧光进行视网膜RPE成像的不同方法，与单光子激发的荧光眼底镜相比，提供了固有的三维分辨率、更大的传感深度和减少的光损伤。基于传统扫描激光检眼镜(HRT，Heidelberg Engineering，德国)的结构，设计了一种双光子显微镜，用于对死后人类供体眼的视网膜样本进行自发荧光成像。我们能够以视频速率显示单个RPE脂褐素颗粒，展示了将该方法发展到临床实践的潜力，用于像AMD这样的RPE相关视网膜疾病患者。
Age-related macular degeneration (AMD) is among the major concerns in ophthalmology, as it is the primary cause for irreversible blindness in developed countries. Nevertheless, there is poor understanding of the origins and mechanisms that trigger this important ocular disease. In common clinical pratice, AMD is monitored by autofluorescence imaging of the retinal pigment epithelial (RPE) cells through a confocal scanning laser ophthalmoscope. The RPE cells derive their dominant autofluorescence from the lipofuscin granules that accumulate in the cytoplasm with increasing age and disease. We explored a different approach to retinal RPE imaging using two-photon excited autofluorescence, offering intrinsic three-dimensional resolution, larger sensing depth and reduced photodamage compared to single-photon excited fluorescence ophthalmoscopy. A two-photon microscope, based on the architecture of a conventional scanning laser ophthalmoscope (HRT, Heidelberg Engineering, Germany), was designed for autofluorescence imaging on retina samples from postmortem human-donor eyes. We were able to visualize at video-rate speed single RPE lipofuscin granules, demonstrating the potential to develop this method toward clinical practice for patients with RPE-related retinal disease like AMD.
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影响因子：
4.4
作者：
F. Delori;D. Goger;C. Dorey
通讯作者：
F. Delori;D. Goger;C. Dorey
视网膜光损伤减少了视网膜色素上皮中的自发荧光色素沉积。
影响因子：
4.4
作者：
Katz,ML;Eldred,GE
通讯作者：
Eldred,GE
影响因子：
4.4
作者：
J. Sparrow;Koji Nakanishi;C. Parish
通讯作者：
J. Sparrow;Koji Nakanishi;C. Parish
影响因子：
4.4
作者：
F. Delori;M. Fleckner;D. Goger;J. Weiter;C. Dorey
通讯作者：
F. Delori;M. Fleckner;D. Goger;J. Weiter;C. Dorey
影响因子：
56.9
作者：
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者：
WEBB, WW