Diverse secondary metabolites are expressed in particle-associated and free-living microorganisms of the permanently anoxic Cariaco Basin.
Diverse secondary metabolites are expressed in particle-associated and free-living microorganisms of the permanently anoxic Cariaco Basin.
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DOI：
10.1038/s41467-023-36026-w
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发表时间：
2023-02-06
影响因子：
16.6
通讯作者：
Pachiadaki, Maria
中科院分区：
文献类型：
--
作者：
Geller-McGrath, David;Mara, Paraskevi;Taylor, Gordon T. T.;Suter, Elizabeth;Edgcomb, Virginia;Pachiadaki, Maria
次生代谢物在生态相互作用和营养获取中起着重要作用，并因其在医学和生物技术中的潜在用途而受到关注。生物合成基因簇（BGC）的基因组挖掘可用于发现新化合物。在这里，我们使用宏基因组学和元转录组学分析BGC在自由生活和颗粒相关的微生物群落通过分层水柱的Cariaco盆地，委内瑞拉。我们回收了565个细菌和古细菌宏基因组组装的基因组（MAG），并确定了1154个不同的BGC。我们发现，水的氧化还原电位和微生物的生活方式（颗粒相关与自由生活）的差异与预测的组成和生产的次生代谢产物的变化。我们的研究结果表明，微生物，包括未充分研究的分支，如浮游菌门，可能会产生广泛的次生代谢产物在这些缺氧/缺氧沃茨。生物合成基因簇（BGC）的基因组挖掘可用于发现生物技术感兴趣的新化合物。在这里，作者使用宏基因组学和元转录组学通过委内瑞拉Cariaco盆地的分层水柱来识别自由生活和颗粒相关微生物群落中的不同BGC。
Secondary metabolites play essential roles in ecological interactions and nutrient acquisition, and are of interest for their potential uses in medicine and biotechnology. Genome mining for biosynthetic gene clusters (BGCs) can be used for the discovery of new compounds. Here, we use metagenomics and metatranscriptomics to analyze BGCs in free-living and particle-associated microbial communities through the stratified water column of the Cariaco Basin, Venezuela. We recovered 565 bacterial and archaeal metagenome-assembled genomes (MAGs) and identified 1154 diverse BGCs. We show that differences in water redox potential and microbial lifestyle (particle-associated vs. free-living) are associated with variations in the predicted composition and production of secondary metabolites. Our results indicate that microbes, including understudied clades such as Planctomycetota, potentially produce a wide range of secondary metabolites in these anoxic/euxinic waters. Genome mining for biosynthetic gene clusters (BGCs) can be used for the discovery of new compounds of biotechnological interest. Here, the authors use metagenomics and metatranscriptomics to identify diverse BGCs in free-living and particle-associated microbial communities through the stratified water column of the Cariaco Basin, Venezuela.
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影响因子：
5.5
作者：
Benjdia A;Balty C;Berteau O
通讯作者：
Berteau O
影响因子：
9.5
作者：
Blin K;Kim HU;Medema MH;Weber T
通讯作者：
Weber T
影响因子：
3.5
作者：
Argueta, Erick A.;Amoh, Amanda N.;Schneider, Tanya L.
通讯作者：
Schneider, Tanya L.
影响因子：
9.2
作者：
Castelle, Cindy J.;Wrighton, Kelly C.;Banfield, Jillian F.
通讯作者：
Banfield, Jillian F.
DOI：
10.3390/antibiotics7010012
发表时间：
2018-02-13
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Choudoir MJ;Pepe-Ranney C;Buckley DH
通讯作者：
Buckley DH