Cytotoxic hydrophilic iminophosphorane coordination compounds of d⁸ metals. Studies of their interactions with DNA and HSA.
Cytotoxic hydrophilic iminophosphorane coordination compounds of d⁸ metals. Studies of their interactions with DNA and HSA.
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DOI：
10.1016/j.jinorgbio.2012.06.017
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发表时间：
2012-11
影响因子：
3.9
通讯作者：
Contel, Maria
中科院分区：
文献类型：
--
作者：
Carreira, Monica;Calvo-Sanjuan, Ruben;Sanau, Mercedes;Zhao, Xiangbo;Magliozzo, Richard S.;Marzo, Isabel;Contel, Maria
The synthesis and characterization of a new water-soluble N,N-chelating iminophosphorane ligand TPA=N-C(O)-2-NC5H4 (N,N-IM) (1) and its d8 (AuIII, PdII and PtII) coordination complexes are reported. The structures of cationic [AuCl2(N,N-IM)] ClO4 (2) and neutral [MCl2(N,N-IM)] M = Pd (3), Pt(4) complexes were determined by X-ray diffraction studies or by means of density-functional calculations. While the Pd and Pt compounds are stable in mixtures of DMSO/H2O over 4 days, the gold derivative (2) decomposes quickly to TPA=O and previously reported neutral gold(III) compound [AuCl2(N,N-H)] 5 (containing the chelating N,N- fragment HN-C(O)-2-NC5H4). The cytotoxicities of complexes 2–5 were evaluated in vitro against human Jurkat-T acute lymphoblastic leukemia cells and DU-145 human prostate cancer cells. Pt (4) and Au compounds (2 and 5) are more cytotoxic than cisplatin to these cell lines and to cisplatin-resistant Jurkat sh-Bak cell lines and their cell death mechanism is different from that of cisplatin. All the compounds show higher toxicity against leukemia cells when compared to normal human T-lymphocytes (PBMC). The interaction of the Pd and Pt compounds with calf thymus and plasmid (pBR322) DNA is different from that of cisplatin. All compounds bind to human serum albumin (HSA) faster than cisplatin (measured by fluorescence spectroscopy). Weak and stronger binding interactions were found for the Pd (3) and Pt (4) derivatives by isothermal titration calorimetry. Importantly, for the Pt (4) compounds the binding to HSA was reversed by addition of a chelating agent (citric acid) and by a decrease in pH.
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影响因子：
3.6
作者：
BITTNER, S;ASSAF, Y;SMITH, CG
通讯作者：
SMITH, CG
影响因子：
3.3
作者：
Falvello, LR;García, MM;Urriolabeitia, EP
通讯作者：
Urriolabeitia, EP
影响因子：
2.3
作者：
Elie, Benelita T.;Levine, Chaya;Ubarretxena-Belandia, Iban;Varela-Ramirez, Armando;Aguilera, Renato J.;Ovalle, Rafael;Contel, Maria
通讯作者：
Contel, Maria
有机金属抗癌化合物。
影响因子：
7.3
作者：
Gasser G;Ott I;Metzler-Nolte N
通讯作者：
Metzler-Nolte N
影响因子：
3.9
作者：
BLANK, CE;DABROWIAK, JC
通讯作者：
DABROWIAK, JC