Improved tolerance to various abiotic stresses in transgenic sweet potato (Ipomoea batatas) expressing spinach betaine aldehyde dehydrogenase.
Improved tolerance to various abiotic stresses in transgenic sweet potato (Ipomoea batatas) expressing spinach betaine aldehyde dehydrogenase.
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表达菠菜甜菜碱醛脱氢酶的转基因甘薯（Ipomoea batatas）对各种非生物胁迫的耐受性提高
DOI：
10.1371/journal.pone.0037344
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang P
中科院分区：
文献类型：
--
作者：
Fan W;Zhang M;Zhang H;Zhang P
非生物胁迫是甘薯（Ipomoea batatas）提高生产力和扩大种植的关键因素，甘薯是一种具有世界重要性的块根作物。甜菜碱（GB）产量的增加提高了植物对各种非生物胁迫的耐受性，而没有强烈的表型变化，为在不利条件下提高稳定产量生产提供了可行的途径。甜菜碱醛脱氢酶（BADH）基因参与植物中甜菜碱醛的生物合成，通过异源过量表达BADH积累的甜菜碱醛提高了植物对非生物胁迫的耐受性。本研究旨在通过促进甜菜碱积累菌甘薯的生物合成来提高甘薯对多种非生物胁迫的抗性。利用农杆菌介导法将菠菜叶绿体BADH基因（SoBADH）导入甘薯品种苏薯2号。转基因甘薯中SoBADH的过表达提高了对各种非生物胁迫的耐受性，包括盐、氧化胁迫和低温。在正常和多种环境胁迫下，转基因植物株系中BADH活性和GB积累的增加导致通过维持细胞膜完整性、增强光合活性、减少活性氧（ROS）产生以及通过增加自由基清除酶的活性诱导或激活ROS清除来增加对细胞损伤的保护。胁迫处理后转基因植株中脯氨酸积累增加和许多ROS清除基因的系统上调也表明GB积累可能通过整合机制刺激ROS清除系统和脯氨酸生物合成。本研究表明，提高甘薯中GB的生物合成是提高甘薯对多种非生物胁迫的耐受性而不引起表型缺陷的有效可行的途径。这种甘薯性状改良策略不仅可以在不可预测的气候条件下稳定正常土壤的产量，而且还为边际土地的甘薯生产提供了新的种质。
Abiotic stresses are critical delimiters for the increased productivity and cultivation expansion of sweet potato (Ipomoea batatas), a root crop with worldwide importance. The increased production of glycine betaine (GB) improves plant tolerance to various abiotic stresses without strong phenotypic changes, providing a feasible approach to improve stable yield production under unfavorable conditions. The gene encoding betaine aldehyde dehydrogenase (BADH) is involved in the biosynthesis of GB in plants, and the accumulation of GB by the heterologous overexpression of BADH improves abiotic stress tolerance in plants. This study is to improve sweet potato, a GB accumulator, resistant to multiple abiotic stresses by promoted GB biosynthesis. A chloroplastic BADH gene from Spinacia oleracea (SoBADH) was introduced into the sweet potato cultivar Sushu-2 via Agrobacterium-mediated transformation. The overexpression of SoBADH in the transgenic sweet potato improved tolerance to various abiotic stresses, including salt, oxidative stress, and low temperature. The increased BADH activity and GB accumulation in the transgenic plant lines under normal and multiple environmental stresses resulted in increased protection against cell damage through the maintenance of cell membrane integrity, stronger photosynthetic activity, reduced reactive oxygen species (ROS) production, and induction or activation of ROS scavenging by the increased activity of free radical-scavenging enzymes. The increased proline accumulation and systemic upregulation of many ROS-scavenging genes in stress-treated transgenic plants also indicated that GB accumulation might stimulate the ROS-scavenging system and proline biosynthesis via an integrative mechanism. This study demonstrates that the enhancement of GB biosynthesis in sweet potato is an effective and feasible approach to improve its tolerance to multiple abiotic stresses without causing phenotypic defects. This strategy for trait improvement in sweet potato not only stabilizes yield production in normal soils in unpredictable climates but also provides a novel germplasm for sweet potato production on marginal lands.
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影响因子：
7.4
作者：
Huang, J;Hirji, R;Selvaraj, G
通讯作者：
Selvaraj, G
影响因子：
7.4
作者：
Battaglia, Marina;Olvera-Carrillo, Yadira;Covarrubias, Alejandra A.
通讯作者：
Covarrubias, Alejandra A.
影响因子：
13.8
作者：
Kathuria, Hitesh;Giri, Jitender;Tyagi, Akhilesh K.
通讯作者：
Tyagi, Akhilesh K.
DOI：
10.1073/pnas.0604882103
发表时间：
2006-08-29
影响因子：
11.1
作者：
Hu, Honghong;Dai, Mingqiu;Xiong, Lizhong
通讯作者：
Xiong, Lizhong
影响因子：
4.3
作者：
Allakhverdiev, SI;Murata, N
通讯作者：
Murata, N