The Inhibition of Ocean Acidification on the Formation of Oyster Calcified Shell by Regulating the Expression of Cgchs1 and Cgchit4
The Inhibition of Ocean Acidification on the Formation of Oyster Calcified Shell by Regulating the Expression of Cgchs1 and Cgchit4
复制标题
海洋酸化调节Cgchs1和Cgchit4表达抑制牡蛎钙化壳的形成
DOI：
10.3389/fphys.2019.01034
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发表时间：
2019-08
影响因子：
4
通讯作者：
Song Linsheng
中科院分区：
文献类型：
--
作者：
Zhang Yukun;Liu Zhaoqun;Song Xiaorui;Huang Shu;Wang Lingling;Song Linsheng
生物合成的钙化壳是牡蛎幼虫发育的关键。这一过程可被CO2引起的海洋酸化严重抑制，导致牡蛎幼虫大量死亡。然而，这一过程的分子机制至今尚未得到很好的研究。本研究从太平洋牡蛎（Crassostrea gigas）中鉴定了一个几丁质合成酶的同源物（命名为Cgchs1）和一个几丁质酶的同源物（命名为Cgchit4）。Cgchs1和Cgchit4的cDNA序列分别为813 bp和2118 bp，分别编码271个氨基酸和706个氨基酸。Cgchs1和Cgchit4的推测氨基酸序列中分别含有一个Chitin_18_2结构域和一个Glyco_18结构域。Cgchs1和Cgchit4与它们在脊椎动物中的同源物具有高度的序列同一性。此外，当牡蛎幼体暴露于酸化处理（pH 7.4）时，其贝壳生物合成过程受到严重抑制。酸化处理显著抑制了Cgchs1 mRNA的表达，而激活了Cgchit4 mRNA的表达。Cgchs1和Cgchit4是甲壳素代谢的关键酶，其mRNA表达的变化可能导致牡蛎幼体壳中甲壳素含量的降低，从而导致成壳失败。因此，酸化海水可能通过抑制甲壳素的生物合成来抑制牡蛎钙化壳的形成。
The biosynthesis of a calcified shell is critical for the development of oyster larvae. This process can be severely inhibited by CO2-induced ocean acidification, causing mass mortality of oyster larvae. However, the underlying molecular mechanism of such process has not been well explored until now. In the present study, a homolog of chitin synthase (named as Cgchs1) and a homolog of chitinase (named as Cgchit4) were identified from the Pacific oyster Crassostrea gigas. The cDNA sequences of Cgchs1 and Cgchit4 were of 813 bp and 2118 bp, encoding a putative polypeptide of 271 amino acids and 706 amino acids, respectively. There were a Chitin_synth_2 domain and a Glyco_18 domain in the inferred amino acid sequences of Cgchs1 and Cgchit4, respectively. Both Cgchs1 and Cgchit4 shared high sequence identity with their homologs in vertebrates. In addition, when oyster larvae were exposed to acidification treatment (pH 7.4), their shell biosynthesis process was seriously restrained. The expression level of Cgchs1 mRNA was significantly suppressed while that of Cgchit4 was dramatically activated upon acidification treatment. Cgchs1 and Cgchit4 are critical enzymes for chitin metabolism, and such changes in their mRNA expression could result in the decrease of chitin content in oyster larvae’s shells, which might lead to the failure of shell formation. Therefore, results in the present study suggested that acidified seawater might inhibit the formation of oyster calcified shell by suppressing the biosynthesis of chitin.
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影响因子：
2.9
作者：
Brahim Mali;F. Möhrlen;M. Frohme;U. Frank
通讯作者：
Brahim Mali;F. Möhrlen;M. Frohme;U. Frank
影响因子：
3
作者：
Wendl MC
通讯作者：
Wendl MC
在高二氧化碳排放的世界中，贝类面临着不确定的未来：酸化对河口牡蛎幼虫钙化和生长的影响。
影响因子：
3.7
作者：
Miller AW;Reynolds AC;Sobrino C;Riedel GF
通讯作者：
Riedel GF
影响因子：
4.8
作者：
RENKEMA, GH;BOOT, RG;AERTS, JMFG
通讯作者：
AERTS, JMFG
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen