Carrot cell growth response in a stimulated ultrasonic environment

Carrot cell growth response in a stimulated ultrasonic environment
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受刺激的超声波环境中胡萝卜细胞的生长反应

DOI:
10.1016/s0927-7765(98)00061-7
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发表时间:
1998-11
期刊:
Colliods and surfaces B;Bioint
影响因子:
--
通讯作者:
Wang Bochu etal
Wang Bochu etal
中科院分区:
其他
文献类型:
--
作者:
Wang Bochu etal

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将悬浮的胡萝卜细胞暴露于28 kHz连续波超声。在细胞悬浮培养开始时,暴露持续时间为2至40 s,以便涵盖每个作用时间的前十年存活率降低或增加。本实验采用分光光度计直接测量悬浮培养中细胞吸光度的变化来检测细胞生长情况。实验结果表明,随着超声作用时间的变化,对悬浮培养的植物细胞生长的影响是积极的还是消极的;同时,我们得到了往复振荡器和旋转振荡器两种振荡方式,在指定的条件下,它们对植物细胞生长的影响几乎相同。
Carrot cells were exposed in suspension to 28 kHz continuous wave ultrasound. Exposure durations were from 2 to 40 s at the beginning of the cell suspension culture, so as to encompass the first decade of decreased or increased survival at each action time. In our experiment, we used a spectrophotometer to assay cell growth by means of measurement directly the absorbance (ABS) change in suspension culture. The experimental results indicated whether it would have been a positive or negative influence on plant cell growth in suspension culture with ultrasound exposed time varying; Meanwhile, we got two shake modes, a reciprocal shaker and a rotary shaker, that had almost same influences on plant cell growth under assigned conditions.
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发表时间: 1966-07
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影响因子: 64.8
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