Fluorouracil (5-FU) and cytarabine (ara-C) inhibition of corneal epithelial cell and conjunctival fibroblast proliferation.
Fluorouracil (5-FU) and cytarabine (ara-C) inhibition of corneal epithelial cell and conjunctival fibroblast proliferation.
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氟尿嘧啶 (5-FU) 和阿糖胞苷 (ara-C) 抑制角膜上皮细胞和结膜成纤维细胞增殖。
DOI：
10.1001/archopht.1985.01050090150052
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发表时间：
1985
影响因子：
--
通讯作者：
R. Parrish
中科院分区：
文献类型：
--
作者：
K. Mallick;A. Hajek;R. Parrish
在兔角膜上皮细胞和结膜成纤维细胞的组织培养模型中，我们评价了氟尿嘧啶(5-FU)和阿糖胞苷(Ara-C)的抗增殖作用。用氟尿嘧啶或阿糖胞苷处理细胞5天。0.6 mg/L氟尿嘧啶或30微克/L阿糖胞苷可使角膜上皮细胞增殖抑制至对照组的50%。氟尿嘧啶0.5 mg/L或阿糖胞苷50微克/L对结膜成纤维细胞的增殖抑制作用为对照组的50%。胸腺嘧啶核苷不阻断10 mg/L氟尿嘧啶的抗增殖作用。浓度分别为10(-5)M和10(-4)M的2‘-脱氧胞苷可阻断100或1000微克/L阿糖胞苷对细胞增殖的抑制作用。氟尿嘧啶和阿糖胞苷对两种细胞类型的抗增殖作用相似。2‘-脱氧胞苷可阻断阿糖胞苷相关的眼毒性作用。
We evaluated the antiproliferative properties of fluorouracil (5-FU) and cytarabine (ara-C) in a tissue culture model of rabbit corneal epithelial cells and conjunctival fibroblasts. Cells were treated with fluorouracil or cytarabine for five days. Corneal epithelial cell proliferation was inhibited to 50% of control proliferation by 0.6 mg/L of fluorouracil or 30 micrograms/L of cytarabine. Proliferation of conjunctival fibroblasts was inhibited to 50% of control by 0.5 mg/L of fluorouracil or 50 micrograms/L of cytarabine. The antiproliferative effect of 10 mg/L of fluorouracil was not blocked by thymidine. The inhibition of proliferation produced by 100 or 1,000 micrograms/L of cytarabine was blocked by 2'-deoxycytidine at concentrations of 10(-5) M and 10(-4) M, respectively. The antiproliferative effects of fluorouracil and cytarabine appear similar for both cell types. Blockage of cytarabine-related ocular toxic effects may be possible with 2'-deoxycytidine.
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影响因子：
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作者：
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影响因子：
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