Integration of RNAi and RNA-seq Reveals the Immune Responses of Epinephelus coioides to sigX Gene of Pseudomonas plecoglossicida.
Integration of RNAi and RNA-seq Reveals the Immune Responses of Epinephelus coioides to sigX Gene of Pseudomonas plecoglossicida.
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RNAi 和 RNA-seq 整合揭示斜带石斑鱼对杀异舌假单胞菌 sigX 基因的免疫反应
DOI：
10.3389/fimmu.2018.01624
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发表时间：
2018
影响因子：
7.3
通讯作者：
Yan Q
中科院分区：
文献类型：
--
作者：
Sun Y;Luo G;Zhao L;Huang L;Qin Y;Su Y;Yan Q
绒舌假单胞菌是一种重要的水产致病菌，在多种海鱼中具有很高的致死率。通过实时荧光定量PCR （qRT-PCR）证实，在18°C时sigX的表达比在28°C时明显上调。RNAi在体外和脾脏各采样时间点均显著降低了ppleclossisidida sigX mRNA的含量。与野生型菌株相比，sigX-RNAi菌株感染可使石斑鱼发病时间延迟，死亡率降低20%，石斑鱼脾脏症状减轻。与野生型菌株相比，sigX基因沉默导致感染的大肠杆菌转录组发生显著变化。基因本体论和KEGG分析结果显示，丝氨酸型内多肽酶及趋化因子信号通路、凝血补体系统、IgA产生途径的肠道免疫网络等基因受sigX的影响最大。同时，免疫基因与不同数量的miRNA和lncRNA相关，部分miRNA同时与多个基因相关。结果表明，sigX是pplecogssisidida的毒力基因。免疫途径的上调使得大肠杆菌比野生型菌株更容易杀死sigX-RNAi菌株，而免疫基因受miRNA和lncRNA的复杂模式调控。
Pseudomonas plecoglossicida is an important pathogen for aquaculture and causes high mortality in various marine fishes. Expression of sigX was found significantly up-regulated at 18°C than at 28°C, which was verified by quantitative real-time PCR (qRT-PCR). RNAi significantly reduced the content of sigX mRNA of P. plecoglossicida, whether in in vitro or in the spleen at all sampling time points. Compared with the wild-type strain, the infection of sigX-RNAi strain resulted in the onset time delay, and 20% reduction in mortality of Epinephelus coioides, as well as alleviates in the symptoms of E. coioides spleen. Compared with wild-type strain, the gene silence of sigX in P. plecoglossicida resulted in a significant change in transcriptome of infected E. coioides. The result of gene ontology and KEGG analysis on E. coioides showed that genes of serine-type endopeptidase and chemokine signaling pathway, coagulation and complement system, and intestinal immune network for IgA production pathway were mostly affected by sigX of P. plecoglossicida. Meanwhile, the immune genes were associated with different number of miRNA and lncRNA, and some miRNAs were associated with more than one gene at the same time. The results indicated that sigX was a virulent gene of P. plecoglossicida. The up-regulation of the immune pathways made E. coioides more likely to kill sigX-RNAi strain than the wild-type strain of P. plecoglossicida, while the immune genes were regulated by miRNA and lncRNA by a complex mode.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
在伤口修复的早期增殖阶段暴露于TGF -β的成纤维细胞的生物信息学分析。
影响因子：
3.4
作者：
Mi B;Liu G;Zhou W;Lv H;Zha K;Liu Y;Wu Q;Liu J
通讯作者：
Liu J
影响因子：
2.4
作者：
Bhaskaran M;Mohan M
通讯作者：
Mohan M
影响因子：
9
作者：
Oikonomopoulou, Katerina;Ricklin, Daniel;Ward, Peter A.;Lambris, John D.
通讯作者：
Lambris, John D.
影响因子：
3.3
作者：
Duchesne, Rachel;Bouffartigues, Emeline;Chevalier, Sylvie
通讯作者：
Chevalier, Sylvie