Cryo-electron tomography of bacteria: progress, challenges and future prospects.

Cryo-electron tomography of bacteria: progress, challenges and future prospects.
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DOI:
10.1038/nrmicro2183
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发表时间:
2009-09
期刊:
Nature reviews. Microbiology
影响因子:
--
通讯作者:
Subramaniam S
Subramaniam S
中科院分区:
其他
文献类型:
--
作者:
Milne JL;Subramaniam S

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Recent advances in three-dimensional electron microscopy provide remarkable tools to image the interior of bacterial cells. Glimpses of cells at resolutions that are 1–2 orders of magnitude higher than those currently attained with light microscopy can now be obtained with cryo-electron tomography, especially when used in combination with new tools for image averaging. This Review highlights recent advances in this area and provides an assessment of the general applicability, current limitations and type of structural information that can be obtained about the organization of intact cells using tomography. Possible future directions for whole cell imaging are also discussed.
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