Kaempferol 3-O-rhamnoside-7-O-rhamnoside is an endogenous flavonol inhibitor of polar auxin transport in Arabidopsis shoots.
Kaempferol 3-O-rhamnoside-7-O-rhamnoside is an endogenous flavonol inhibitor of polar auxin transport in Arabidopsis shoots.
复制标题
DOI：
10.1111/nph.12558
复制
发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Schäffner AR
中科院分区：
文献类型：
--
作者：
Yin R;Han K;Heller W;Albert A;Dobrev PI;Zažímalová E;Schäffner AR
植物生长素极性转运（PAT）在植物生长发育调控中起着关键作用。黄酮类化合物与抑制PAT有关。然而，体内参与这一过程的活性类黄酮衍生物尚未被确定。在这里，我们提供的证据表明，一种特定的黄酮醇双糖苷与较短的植物身高和降低的PAT有关。拟南芥中特定的类黄酮生物合成或类黄酮糖基化步骤被遗传阻断。采用高效液相色谱法对所建立的黄酮醇差异谱进行了分析，并分析了其与植物株高变化的关系。使用放射性[3H]-吲哚-3-乙酸示踪剂监测茎段中的PAT。类黄酮3- o -葡萄糖基转移酶突变体ugt78d2除了黄酮醇苷模式改变外，还表现出矮化的身材。这伴随着ugt78d2芽中PAT的减少。ugt78d2依赖性生长缺陷是类黄酮依赖性生长缺陷，可通过基因阻断类黄酮生物合成来修复。对一系列在黄酮类化合物形成的不同步骤中存在缺陷的突变体的表型和代谢分析将潜在的活性片段缩小到山奈酚3- o -鼠李糖苷-7- o -鼠李糖苷。此外，该化合物的水平与基部生长素运输呈负相关。这些结果表明山奈酚3- o -鼠李糖苷-7- o -鼠李糖苷在拟南芥芽中具有内源性PAT抑制剂的作用。
Polar auxin transport (PAT) plays key roles in the regulation of plant growth and development. Flavonoids have been implicated in the inhibition of PAT. However, the active flavonoid derivative(s) involved in this process in vivo has not yet been identified. Here, we provide evidence that a specific flavonol bis-glycoside is correlated with shorter plant stature and reduced PAT. Specific flavonoid-biosynthetic or flavonoid-glycosylating steps were genetically blocked in Arabidopsis thaliana. The differential flavonol patterns established were analyzed by high-performance liquid chromatography (HPLC) and related to altered plant stature. PAT was monitored in stem segments using a radioactive [3H]-indole-3-acetic acid tracer. The flavonoid 3-O-glucosyltransferase mutant ugt78d2 exhibited a dwarf stature in addition to its altered flavonol glycoside pattern. This was accompanied by reduced PAT in ugt78d2 shoots. The ugt78d2-dependent growth defects were flavonoid dependent, as they were rescued by genetic blocking of flavonoid biosynthesis. Phenotypic and metabolic analyses of a series of mutants defective at various steps of flavonoid formation narrowed down the potentially active moiety to kaempferol 3-O-rhamnoside-7-O-rhamnoside. Moreover, the level of this compound was negatively correlated with basipetal auxin transport. These results indicate that kaempferol 3-O-rhamnoside-7-O-rhamnoside acts as an endogenous PAT inhibitor in Arabidopsis shoots.
登录
查看更多内容
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
7.4
作者：
Murphy, AS;Hoogner, KR;Taiz, L
通讯作者：
Taiz, L
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.2
作者：
Mathesius, U;Schlaman, HRM;Djordjevic, MA
通讯作者：
Djordjevic, MA
影响因子：
56.9
作者：
JACOBS, M;RUBERY, PH
通讯作者：
RUBERY, PH