A high-fat high-energy diet influences hepatic CYP3A expression and activity in low-birth-weight developing female rats
A high-fat high-energy diet influences hepatic CYP3A expression and activity in low-birth-weight developing female rats
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高脂肪高能量饮食影响低出生体重雌性大鼠肝脏 CYP3A 表达和活性
DOI：
10.1007/s12519-016-0019-9
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
倪韶青
中科院分区：
文献类型：
--
作者：
倪韶青
本研究旨在探讨高脂高能饲料对低出生体重发育雌性大鼠肝脏细胞色素P3A(CyP3A)表达的影响。营养不良大鼠的子代定义为正常出生体重(NBW)组，营养不良大鼠的子代定义为低出生体重(LBW)组。根据出生体重和饮食将大鼠分为四组：NBW-正常饮食(NN)组、NBW-HFHE(NH)组、LBW-正常饮食(LN)组和LBW-HFHE(LH)组。结果出生后第3、56、84天，黄体生成素组大鼠肝脏细胞色素P3A1基因表达水平显著高于正常对照组(P<0.05)。在第21、28、84天，黄体生成素组细胞色素P3A1的表达均显著高于非黄体组(P<0.05)。在第3天和第21天，黄体生成素组细胞色素P3A1基因的表达均高于黄体生成素组(P<0.05)。促黄体生成素发育组未观察到细胞色素P3A1的带状表达模式。在第7、14、28和56天，黄体生成素组的平均活性显著高于黄体生成素组。结论高脂膳食可导致发育期LBW大鼠模型细胞色素P3A表达的改变。
BackgroundThe objective of this study was to investigate the effects of a high-fat, high-energy (HFHE) diet on the hepatic expression of CYP3A in low-birthweight developing female rats.MethodsPregnant rats were divided into nourished and undernourished groups. The offspring of the nourished rats were defined as the normal-birth-weight (NBW) group, and those of undernourished rats were defined as the low-birth-weight (LBW) group. According to their birth weights and diets, the rats were subdivided into the following four groups: NBW-normal diet (NN) group; NBW-HFHE (NH) group; LBW-normal diet (LN) group; and LBW-HFHE (LH) group. Liver samples were isolated on days 3, 7, 14, 21, 28, 56 and 84 after birth.ResultsThe CYP3A1 mRNA levels in the LH group on days 3, 56 and 84 were significantly higher than those of the NN group (P<0.05). CYP3A1 expression was significantly higher in the LH group than that in the NH group on days 21, 28 and 84 (P<0.05). CYP3A1 mRNA expression was higher in the LH group than that in the LN group on days 3 and 21 (P<0.05). No zonal CYP3A1 expression pattern was observed in the LH developmental group. The LH group had significantly higher mean activity than the LN group on days 7, 14, 28 and 56.ConclusionOur results indicated that an HFHE diet can result in alterations of CYP3A expression in a developmental LBW rat model.
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DOI：
10.1042/bj3090055
发表时间：
1995-07
期刊：
The Biochemical journal
影响因子：
--
作者：
T. Oinonen;K. Lindros
通讯作者：
T. Oinonen;K. Lindros
影响因子：
3.7
作者：
Cunha Fda S;Dalle Molle R;Portella AK;Benetti Cda S;Noschang C;Goldani MZ;Silveira PP
通讯作者：
Silveira PP
影响因子：
4.2
作者：
Claycombe, Kate J.;Uthus, Eric O.;Johnson, W. Thomas
通讯作者：
Johnson, W. Thomas
DOI：
10.1016/j.vascn.2006.07.002
发表时间：
2007-03
影响因子：
1.9
作者：
J. Michaud;F. Leblond;Judith Naud;C. Boisvert;K. Desbiens;D. Nicoll-Griffith;V. Pichette
通讯作者：
J. Michaud;F. Leblond;Judith Naud;C. Boisvert;K. Desbiens;D. Nicoll-Griffith;V. Pichette
影响因子：
6.1
作者：
Ghose, Romi;Omoluabi, Ozozoma;Gandhi, Adarsh;Shah, Pranav;Strohacker, Kelley;Carpenter, Katie C.;McFarlin, Brian;Guo, Tao
通讯作者：
Guo, Tao