Evidence for multiple, distinct ADAR-containing complexes in Xenopus laevis.
Evidence for multiple, distinct ADAR-containing complexes in Xenopus laevis.
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DOI：
10.1261/rna.047787.114
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Blower MD
中科院分区：
文献类型：
--
作者：
Schweidenback CT;Emerman AB;Jambhekar A;Blower MD
ADAR (adenosine deaminase acting on RNA) is an RNA-editing enzyme present in most metazoans that converts adenosines in double-stranded RNA targets into inosines. Although the RNA targets of ADAR-mediated editing have been extensively cataloged, our understanding of the cellular function of such editing remains incomplete. We report that long, double-stranded RNA added to Xenopus laevis egg extract is incorporated into an ADAR-containing complex whose protein components resemble those of stress granules. This complex localizes to microtubules, as assayed by accumulation on meiotic spindles. We observe that the length of a double-stranded RNA influences its incorporation into the microtubule-localized complex. ADAR forms a similar complex with endogenous RNA, but the endogenous complex fails to localize to microtubules. In addition, we characterize the endogenous, ADAR-associated RNAs and discover that they are enriched for transcripts encoding transcriptional regulators, zinc-finger proteins, and components of the secretory pathway. Interestingly, association with ADAR correlates with previously reported translational repression in early embryonic development. This work demonstrates that ADAR is a component of two, distinct ribonucleoprotein complexes that contain different types of RNAs and exhibit diverse cellular localization patterns. Our findings offer new insight into the potential cellular functions of ADAR.
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影响因子：
3.5
作者：
Borchert GM;Gilmore BL;Spengler RM;Xing Y;Lanier W;Bhattacharya D;Davidson BL
通讯作者：
Davidson BL
影响因子：
11.4
作者：
Ferrandon, D;Koch, I;NussleinVolhard, C
通讯作者：
NussleinVolhard, C
影响因子：
7.8
作者：
Eckmann, CR;Jantsch, MF
通讯作者：
Jantsch, MF
影响因子：
14.9
作者：
Capshew CR;Dusenbury KL;Hundley HA
通讯作者：
Hundley HA
DOI：
10.1083/jcb.200705163
发表时间：
2007-12-31
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blower MD;Feric E;Weis K;Heald R
通讯作者：
Heald R