DasR positively controls monensin production at two-level regulation in Streptomyces cinnamonensis
DasR positively controls monensin production at two-level regulation in Streptomyces cinnamonensis
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DasR 在肉桂链霉菌中以两级调控正向控制莫能菌素的产生
DOI：
10.1007/s10295-016-1845-4
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发表时间：
2016-10
影响因子：
3.4
通讯作者：
Zhao Guang-Rong
中科院分区：
文献类型：
--
作者：
Zhang Yue;Lin Chun-Yan;Li Xiao-Mei;Tang Zheng-Kun;Qiao Jianjun;Zhao Guang-Rong
The polyether ionophore antibiotic monensin is produced by Streptomyces cinnamonensis and is used as a coccidiostat for chickens and growth-promoting agent for cattle. Monensin biosynthetic gene cluster has been cloned and partially characterized. The GntR-family transcription factor DasR regulates antibiotic production and morphological development in Streptomyces coelicolor and Saccharopolyspora erythraea. In this study, we identified and characterized the two-level regulatory cascade of DasR to monensin production in S. cinnamonensis. Forward and reverse genetics by overexpression and antisense RNA silence of dasR revealed that DasR positively controls monensin production under nutrient-rich condition. Electrophoresis mobility shift assay (EMSA) showed that DasR protein specifically binds to the promoter regions of both pathway-specific regulatory gene monRII and biosynthetic genes monAIX, monE and monT. Semi-quantitative RT-PCR further confirmed that DasR upregulates the transcriptional levels of these genes during monensin fermentation. Subsequently, co-overexpressed dasR with pathway-specific regulatory genes monRI, monRII or monH greatly improved monensin production.
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影响因子：
5.7
作者：
Tumova, Lucie;Pombinho, Antonio R.;Korinek, Vladimir
通讯作者：
Korinek, Vladimir
影响因子：
3.7
作者：
Uguru GC;Mondhe M;Goh S;Hesketh A;Bibb MJ;Good L;Stach JE
通讯作者：
Stach JE
DOI：
--
发表时间：
2000
期刊：
--
影响因子：
--
作者：
T. Kieser
通讯作者：
T. Kieser
影响因子：
1.2
作者：
C. Wilkinson;Z. Hughes-Thomas;Christine J. Martin;I. Böhm;T. Mironenko;M. Deacon;M. Wheatcroft;G. Wirtz;J. Staunton;P. Leadlay
通讯作者：
C. Wilkinson;Z. Hughes-Thomas;Christine J. Martin;I. Böhm;T. Mironenko;M. Deacon;M. Wheatcroft;G. Wirtz;J. Staunton;P. Leadlay
影响因子：
7.7
作者：
Rigali, Sebastien;Titgemeyer, Fritz;van Wezel, Gilles P.
通讯作者：
van Wezel, Gilles P.