Status of two species of lac insects in the genus Kerria from China based on morphological, cellular, and molecular evidence.
Status of two species of lac insects in the genus Kerria from China based on morphological, cellular, and molecular evidence.
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DOI：
10.1673/031.011.10601
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Chen X;Chen H;Feng Y;He R;Yang Z
本文报道了中国紫胶虫云南紫胶蚧Kerria yunnanensis（Ou and Hong）和云南紫胶蚧Kerria yunnanensis（Ou and Hong）的分类地位。通过比较形态学、细胞学和分子生物学数据，对中国产的四种野生稻（Wang、Yao、Teiu和Liang）进行了比较分析。分支分析表明K. yunnanensis和K. ruralis与其他Kerria属种如K. lacca和K. chinensis。对K.云南白药K. yunnanensis为3A，染色体结构为K = 6 m + 2sm +10 T;核型为3B，染色体结构为K = 8 m +10 T。Kerria ruralis和K.云南种的核型同源性最高，亲缘关系最近。根据核型分析结果，K. sindica和K.紫胶与K. ruralis和K.云南种。Kerria pusana和K. nepalensis聚为姐妹分支，表明这些分类群的亲缘关系密切。对K.而中华绒螯蟹与其他6种不同，形成了一个独立的分支。RAPD分析也表明K. yunnanensis和K. ruralis与Kerria的其它种有明显的差异，但它们没有形成姐妹类群。基于EF 1 α基因的分子分析表明，7种紫胶虫可聚为两大类。第1组K. sindica和K.紫胶树形成一个姐妹分支，是该属的原始成员。第2组K. chinensis形成最早的分叉分支，K. ruralis。其次是Kerria yunnanensis，其次是K. pusana和K.尼泊尔。杂交试验表明，K. yunnanensis和K. sindica，K. yunnanensis和K.紫胶虫可产生第一代幼虫。这表明K。云南种与其它种的亲缘关系较远。形态学、细胞学、分子生物学和杂交结果证实了中国特有种K. yunnanensis和K. ruralis。Kerria ruralis与K. yunnanensis，但与K.虫胶。我国主要的经济种是K. yunnanensis，而在泰国为K. chinensis。缅甸的经济种有K. nepalensis和K. pusana，后者在紫胶生产中使用最广泛。
The taxonomic status of the Chinese lac insects Kerria yunnanensis (Ou and Hong) (Hemiptera: Kerridae) and K. ruralis (Wang, Yao, Teiu and Liang) were analyzed in this paper by comparing morphological, cellular, and molecular data. Cladistic analysis showed K. yunnanensis and K. ruralis to be distinct from other Kerria species such as K. lacca and K. chinensis. The karyotype of K. yunnanensis was 3A and the chromosome structure was K = 6m + 2sm + 10T, while in K. ruralis the karyotype was 3B and the chromosome structure was K = 8m + 10T. Kerria ruralis and K. yunnanensis had the closest relationship among species in the genus as they had the most similar karyotype homology. Based on the karyotype analysis, K. sindica and K. lacca formed a sister group with K. ruralis and K. yunnanensis. Kerria pusana and K. nepalensis were clustered as a sister branch, indicating the close relationship of these taxa. The karyotype of K. chinensis was however, different from the other six species and formed a separate branch. RAPD analysis also showed that K. yunnanensis and K. ruralis had distinct differences from other species of Kerria, although they did not form sister taxa. Molecular analysis based on the EF1α gene using ML, MP, and Mr. Bayes' methods indicated that seven species of lac insects cluster in two major groups. In group 1, K. sindica and K. lacca formed a sister clade and were primitive members of the genus. In group 2, K. chinensis formed the earliest diverging branch followed by K. ruralis. Kerria yunnanensis was the next to diverge followed by the cluster containing K. pusana and K. nepalensis. Hybridization testing showed that crosses neither between K. yunnanensis and K. sindica, nor between K. yunnanensis and K. lacca could produce first generation larvae. This was indicative that K. yunnanensis had a distant genetic relationship from the other species. Morphological, cellular, molecular, and hybridization results confirmed the independent status of the Chinese endemic species K. yunnanensis and K. ruralis. Kerria ruralis was genetically closely related to K. yunnanensis, but relatively far from K. lacca. The main commercial species in China was K. yunnanensis, while in Thailand it was K. chinensis. The commercial species in Myanmar included K. nepalensis and K. pusana, the latter being most widely used in lac production.
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影响因子：
9.5
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M
DOI：
10.1017/s000748530005625x
发表时间：
1923-01-01
期刊：
Bulletin of Entomological Research London
影响因子：
--
作者：
Chamberlin, J. C.
通讯作者：
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影响因子：
5.8
作者：
Huelsenbeck, JP;Ronquist, F
通讯作者：
Ronquist, F
DOI：
10.1017/s0007485300056121
发表时间：
1925-01-01
期刊：
Bulletin of Entomological Research London
影响因子：
--
作者：
Chamberlin, J. C.
通讯作者：
Chamberlin, J. C.
影响因子：
3.3
作者：
FELSENSTEIN, J
通讯作者：
FELSENSTEIN, J