Characterization of insulin-like growth factor receptors in rat anterior pituitary, hypothalamus, and brain.
Characterization of insulin-like growth factor receptors in rat anterior pituitary, hypothalamus, and brain.
复制标题
大鼠垂体前叶、下丘脑和大脑中胰岛素样生长因子受体的表征。
作者：
C. Goodyer;Lucie De Stephano;Wei Hsien Lai;H. Guyda;B. Posner
进行研究以确定胰岛素样生长因子（IGF-I和IGF-II）是否与垂体和大脑中的特异性膜受体结合。从成年雄性Sprague-Dawley大鼠（225-300 g）获得垂体前叶、下丘脑和脑（减去下丘脑），并通过差速离心制备15，000 X g膜。125 I-IGF-I和125 I-IGF-II与所有三种膜制剂的结合是特异性的、时间和温度依赖性的、可逆的，并且随着膜蛋白或标记配体浓度的增加而成比例增加。试验缓冲液的pH值（6.5-8.5）和1 mg/ml杆菌肽的存在与否对特异性结合水平均无显著影响。在所有三种膜制备物中，IGF-II特异性结合比IGF-I所观察到的高3-5倍，并且未标记的IGF-II取代125 I-IGF-I或125 I-IGF-II优于相当浓度的IGF-I。所有三种膜制备物均显示出相似的125 I-胰岛素（1.3-2.2%）低特异性结合和125 I-大鼠GH（小于0.5%）可忽略不计的特异性结合。大鼠垂体前叶、下丘脑和脑组织中特异性IGF和胰岛素受体的存在是IGF和胰岛素参与调节脑和垂体功能的额外证据。
Studies were undertaken to determine whether the insulin-like growth factors (IGF-I and -II), bind to specific membrane receptors in the pituitary and brain. Anterior pituitary glands, hypothalami, and brains (minus hypothalami) were obtained from adult male Sprague-Dawley rats (225-300 g) and 15,000 X g membranes prepared by differential centrifugation. Binding of 125I-IGF-I and 125I-IGF-II to all three membrane preparations was specific, time and temperature dependent, reversible, and increased in proportion to increasing concentrations of membrane protein or labeled ligand. Neither the pH of the assay buffer (6.5-8.5) nor the presence or absence of 1 mg/ml bacitracin had any significant effect on the levels of specific binding. In all three membrane preparations IGF-II specific binding was 3-5 times higher than that observed for IGF-I, and unlabeled IGF-II displaced either 125I-IGF-I or 125I-IGF-II better than comparable concentrations of IGF-I. All three membrane preparations showed similar low specific binding of 125I-insulin (1.3-2.2%) and negligible specific binding of 125I-rat GH (less than 0.5%). The presence of specific IGF and insulin receptors in rat anterior pituitary, hypothalamic, and brain tissue is additional evidence that IGFs and insulin are involved in modulating brain and pituitary function.
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生长激素释放的调节：反对大鼠垂体细胞负反馈的证据。
影响因子：
4.8
作者：
Richman,RA;Weiss,JP;Hochberg,Z;Florini,JR
通讯作者：
Florini,JR
DOI：
10.1016/s0021-9258(18)34631-3
发表时间：
1982-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Massagué;M. Czech
通讯作者：
J. Massagué;M. Czech
[生长调节素抑制下丘脑因子生长激素分泌素或合成肽 hpGRF 刺激的生长激素分泌]。
DOI：
--
发表时间：
1982
期刊：
Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie
影响因子：
--
作者：
Brazeau,P;Guillemin,R;Ling,N;vanWyk,J;Humbel,R
通讯作者：
Humbel,R
大鼠碱性生长调节素的分离和部分序列分析。
影响因子：
4.8
作者：
Rubin,JS;Mariz,I;Jacobs,JW;Daughaday,WH;Bradshaw,RA
通讯作者：
Bradshaw,RA
影响因子：
56.9
作者：
BERELOWITZ, M;SZABO, M;HINTZ, RL
通讯作者：
HINTZ, RL