Transcriptomic and phylogenetic analysis of Culex pipiens quinquefasciatus for three detoxification gene families.
Transcriptomic and phylogenetic analysis of Culex pipiens quinquefasciatus for three detoxification gene families.
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三个解毒基因家族的Culex pipiens quinquefasciatus的转录组和系统发育分析。
DOI：
10.1186/1471-2164-13-609
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发表时间：
2012-11-10
期刊：
影响因子：
4.4
通讯作者：
Cui F
中科院分区：
文献类型：
--
作者：
Yan L;Yang P;Jiang F;Cui N;Ma E;Qiao C;Cui F
人类疾病的三种主要蚊媒冈比亚按蚊、埃及伊蚊和致倦库蚊的基因组先前已被测序。C. p. quinquefasciatus具有最大数量的预测蛋白质编码基因，这部分是由于三个解毒基因家族的扩展：细胞色素P450单加氧酶（P450），谷胱甘肽S-转移酶（GST）和羧基/胆碱酯酶（CCE）。但与安不同。gambiae和Ae. aegypti，C.致倦青霉的转录组资源有限。了解完整的基因表达信息对于探索参与特定生物过程的基因的功能是非常重要的。本研究对C.并与其它三种双翅目昆虫进行了系统发育分析。利用数字基因表达（DGE）技术分析了不同发育阶段的基因表达和与抗草甘膦相关的差异表达。共发现302个解毒基因。结果表明，致倦疫霉中存在71个CCE基因、196个P450基因和35个胞浆GST基因。与其他3种双翅目昆虫相比，库蚊基因组的扩增主要发生在CCE和P450家族，其中CYP 4亚家族的α-酯酶、保幼激素酯酶和CYP 325基因的扩增最为明显。对于五个DGE文库，产生了350 - 380万个原始标签并将其映射到13314个参考基因。在302个解毒基因中，225个（75%）在至少一个DGE文库中被检测到表达。四分之一的CCE和P450基因在一个阶段中被检测到，表明潜在的发育调控表达。共有1511个基因在对硫磷抗性品系和敏感品系之间表现出不同的表达水平。15个解毒基因，包括2个CCE，6个GST和7个P450，在抗性菌株中以较高的水平表达。本研究结果为了解蚊体内三个解毒基因家族的功能和进化提供了新的视角，并为蚊体内C. p. quinquefasciatus，这将有助于阐明三种主要蚊媒不同生物学特性的分子机制。
The genomes of three major mosquito vectors of human diseases, Anopheles gambiae, Aedes aegypti, and Culex pipiens quinquefasciatus, have been previously sequenced. C. p. quinquefasciatus has the largest number of predicted protein-coding genes, which partially results from the expansion of three detoxification gene families: cytochrome P450 monooxygenases (P450), glutathione S-transferases (GST), and carboxyl/cholinesterases (CCE). However, unlike An. gambiae and Ae. aegypti, which have large amounts of gene expression data, C. p. quinquefasciatus has limited transcriptomic resources. Knowledge of complete gene expression information is very important for the exploration of the functions of genes involved in specific biological processes. In the present study, the three detoxification gene families of C. p. quinquefasciatus were analyzed for phylogenetic classification and compared with those of three other dipteran insects. Gene expression during various developmental stages and the differential expression responsible for parathion resistance were profiled using the digital gene expression (DGE) technique. A total of 302 detoxification genes were found in C. p. quinquefasciatus, including 71 CCE, 196 P450, and 35 cytosolic GST genes. Compared with three other dipteran species, gene expansion in Culex mainly occurred in the CCE and P450 families, where the genes of α-esterases, juvenile hormone esterases, and CYP325 of the CYP4 subfamily showed the most pronounced expansion on the genome. For the five DGE libraries, 3.5-3.8 million raw tags were generated and mapped to 13314 reference genes. Among 302 detoxification genes, 225 (75%) were detected for expression in at least one DGE library. One fourth of the CCE and P450 genes were detected uniquely in one stage, indicating potential developmentally regulated expression. A total of 1511 genes showed different expression levels between a parathion-resistant and a susceptible strain. Fifteen detoxification genes, including 2 CCEs, 6 GSTs, and 7 P450s, were expressed at higher levels in the resistant strain. The results of the present study provide new insights into the functions and evolution of three detoxification gene families in mosquitoes and comprehensive transcriptomic resources for C. p. quinquefasciatus, which will facilitate the elucidation of molecular mechanisms underlying the different biological characteristics of the three major mosquito vectors.
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DOI：
10.1073/pnas.0505340102
发表时间：
2005-09-27
影响因子：
11.1
作者：
Ishida, Y;Leal, WS
通讯作者：
Leal, WS
影响因子：
3.8
作者：
Ribeiro, JMC;Charlab, R;Valenzuela, JG
通讯作者：
Valenzuela, JG
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.1
作者：
Dunkov, BC;Guzov, VM;Feyereisen, R
通讯作者：
Feyereisen, R
影响因子：
2.6
作者：
Sabourault, C;Guzov, VM;Feyereisen, R
通讯作者：
Feyereisen, R