The Effects of Cucumber Mosaic Virus and Its 2a and 2b Proteins on Interactions of Tomato Plants with the Aphid Vectors Myzus persicae and Macrosiphum euphorbiae.
The Effects of Cucumber Mosaic Virus and Its 2a and 2b Proteins on Interactions of Tomato Plants with the Aphid Vectors Myzus persicae and Macrosiphum euphorbiae.
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DOI：
10.3390/v14081703
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发表时间：
2022-08-01
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影响因子：
--
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中科院分区：
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黄瓜花叶病毒（CMV）是一种以蚜虫为媒介的非持久性番茄病原菌。我们研究了巨细胞病毒诱导的挥发性有机化合物（VOCs）或其他病毒诱导的线索是否会改变蚜虫与番茄的相互作用。y管嗅探结果表明，感染巨细胞病毒（CMV）的植物释放的挥发性有机化合物吸引了多面手（Myzus persicae）和茄科专手（Macrosiphum euphorbiae）蚜虫。在释放后1小时，桃蚜倾向于在接种后3天（dpi）的感染植株上定居，但在接种后9和21 dpi时，蚜虫倾向于在模拟接种植株上定居。在3 dpi时，大大麻对模拟接种植株和侵染植株没有强烈的偏好，但在9 dpi和21 dpi时，大大麻对模拟接种植株的偏好较强。在黑暗中，蚜虫对模拟接种或感染植株均无定居或迁移倾向。然而，番茄VOC混合物在光照和黑暗中不同，这表明蚜虫对受感染影响的嗅觉、视觉和其他线索的复杂组合做出反应。LS-CMV菌株没有引起蚜虫与植物相互作用的变化。利用株间重组病毒和假重组病毒进行的实验表明，Fny-CMV 2a和2b蛋白分别修饰了番茄与大黄蜂和桃蚜的相互作用。防御信号水杨酸可以防止cmv诱导的对番茄植株的过度伤害，但不参与cmv诱导的蚜虫与植株相互作用的变化。
Cucumber mosaic virus (CMV), a major tomato pathogen, is aphid-vectored in the non-persistent manner. We investigated if CMV-induced volatile organic compounds (VOCs) or other virus-induced cues alter aphid–tomato interactions. Y-tube olfactometry showed that VOCs emitted by plants infected with CMV (strain Fny) attracted generalist (Myzus persicae) and Solanaceae specialist (Macrosiphum euphorbiae) aphids. Myzus persicae preferred settling on infected plants (3 days post-inoculation: dpi) at 1h post-release, but at 9 and 21 dpi, aphids preferentially settled on mock-inoculated plants. Macrosiphum euphorbiae showed no strong preference for mock-inoculated versus infected plants at 3 dpi but settled preferentially on mock-inoculated plants at 9 and 21 dpi. In darkness aphids showed no settling or migration bias towards either mock-inoculated or infected plants. However, tomato VOC blends differed in light and darkness, suggesting aphids respond to a complex mix of olfactory, visual, and other cues influenced by infection. The LS-CMV strain induced no changes in aphid–plant interactions. Experiments using inter-strain recombinant and pseudorecombinant viruses showed that the Fny-CMV 2a and 2b proteins modified tomato interactions with Macrosiphum euphorbiae and Myzus persicae, respectively. The defence signal salicylic acid prevents excessive CMV-induced damage to tomato plants but is not involved in CMV-induced changes in aphid–plant interactions.
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