Identification of no pollen 1 provides a candidate gene for heterosis utilization in foxtail millet (Setaria italica L.)
Identification of no pollen 1 provides a candidate gene for heterosis utilization in foxtail millet (Setaria italica L.)
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无花粉1的鉴定为谷子(Setaria italica L.)杂种优势利用提供了候选基因
DOI：
10.1016/j.cj.2021.03.018
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发表时间：
2021-05
期刊：
影响因子：
--
通讯作者：
Xianmin Diao
中科院分区：
文献类型：
--
作者：
Wei Zhang;Hui Zhi;Sha Tang;Haoshan Zhang;Yi Sui;Guanqing Jia;Chuanyin Wu;Xianmin Diao
雄性不育是植物界普遍存在的一种生物学现象，利用雄性不育获得的雄性不育系是重要的商业性杂交种遗传资源。虽然越来越多的雄性不育基因已被确定在水稻（水稻）和拟南芥（拟南芥），一些雄性不育相关基因已被确定在谷子（谷子）。在这项研究中，我们分离出一个雄性不育的甲基磺酸乙酯产生的突变体在谷子，没有花粉1（sinp 1），显示异常的乌比希体，有缺陷的花粉外壁和完全的雄性不育。通过大量分离分析，我们克隆了SiNP 1，并通过CRISPR/Cas9基因组编辑证实了其功能。亚细胞定位结果表明，SiNP 1蛋白主要定位于内质网，且主要在穗部表达。转录组分析表明，在该突变体中存在许多差异表达的基因，其中一些基因编码与碳水化合物代谢、脂肪酸合成、脂类运输和代谢有关的蛋白质，这些蛋白质与花粉壁发育密切相关。代谢物组学分析表明，突变体中黄酮代谢和脂肪酸生物合成受到干扰。SiNP 1基因的鉴定为谷子杂种优势利用提供了候选雄性不育基因，并为进一步研究植物花粉繁殖机制提供了理论依据。
Male sterility is a common biological phenomenon in plant kingdom and has been used to generate male-sterile lines, which are important genetic resources for commercial hybrid seed production. Although increasing numbers of male-sterility genes have been identified in rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana), few male-sterility-related genes have been characterized in foxtail millet (Setaria italica). In this study, we isolated a male-sterile ethyl methanesulfonate-generated mutant in foxtail millet,no pollen 1(sinp1), which displayed abnormal Ubisch bodies, defective pollen exine and complete male sterility. Using bulk segregation analysis, we clonedSiNP1and confirmed its function with CRISPR/Cas9 genome editing.SiNP1encoded a putative glucose-methanol-choline oxidoreductase. Subcellular localization showed that the SiNP1 protein was preferentially localized to the endoplasmic reticulum and was predominantly expressed in panicle. Transcriptome analysis revealed that many genes were differentially expressed in thesinp1mutant, some of which encoded proteins putatively involved in carbohydrate metabolism, fatty acid biosynthesis, and lipid transport and metabolism, which were closely associated with pollen wall development. Metabolome analysis revealed the disturbance of flavonoids metabolism and fatty acid biosynthesis in the mutant. In conclusion, identification ofSiNP1provides a candidate male-sterility gene for heterosis utilization in foxtail millet and gives further insight into the mechanism of pollen reproduction in plants.
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杂种大米可行的男性不育系统：遗传学，生物化学，分子生物学和利用率。
DOI：
10.1186/s12284-014-0013-6
发表时间：
2014-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Huang JZ;E ZG;Zhang HL;Shu QY
通讯作者：
Shu QY
影响因子：
5.6
作者：
Petrussa E;Braidot E;Zancani M;Peresson C;Bertolini A;Patui S;Vianello A
通讯作者：
Vianello A
影响因子：
27.5
作者：
Ma, Xingliang;Zhang, Qunyu;Liu, Yao-Guang
通讯作者：
Liu, Yao-Guang
影响因子：
3.7
作者：
Etxebeste O;Herrero-García E;Cortese MS;Garzia A;Oiartzabal-Arano E;de los Ríos V;Ugalde U;Espeso EA
通讯作者：
Espeso EA
影响因子：
46.9
作者：
Bennetzen, Jeffrey L.;Schmutz, Jeremy;Devos, Katrien M.
通讯作者：
Devos, Katrien M.