Comparative genomic analysis of isoproturon-mineralizing sphingomonads reveals the isoproturon catabolic mechanism.
Comparative genomic analysis of isoproturon-mineralizing sphingomonads reveals the isoproturon catabolic mechanism.
复制标题
异丙隆矿化鞘氨醇单胞菌的比较基因组分析揭示了异丙隆分解代谢机制。
DOI：
10.1111/1462-2920.13413
复制
发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
ong
中科院分区：
文献类型：
--
作者：
Yan Xin;Gu Tao;Yi Zhongquan;Huang Junwei;Liu Xiaowei;Zhang Ji;Xu Xihui;Xin Zhihong;Hong Qing;He Jian;Spain Jim C;Li Shunpeng;Jiang Ji;ong
苯脲类除草剂异丙隆（伊普）在世界范围内的广泛使用引起了人们对其环境命运的极大关注。虽然许多微生物代谢产物已被发现，并已分离出IPU矿化细菌，但伊普催化的分子机制尚未阐明。在这项研究中，完整的基因编码保守的伊普分解代谢途径被发现，基于比较分析的三个IPU矿化鞘单胞菌的基因组和随后的实验验证。完整的基因包括一个新的水解酶基因ddhA，它负责伊普脱甲基产物的尿素侧链的切割;一个独特的苯胺双加氧酶基因簇adoQTA 1A 2BR，它具有广泛的底物范围;和一个诱导型儿茶酚间位切割途径基因簇adoXEGKLIJC。进一步证实了起始单N-去甲基化基因pdmAB参与了伊普单N-去甲基化产物的后续N-去甲基化。这些IPU分解代谢基因被组织成四个转录单位，分布在三个质粒上。它们两侧有多个移动的遗传元件，并且在IPU矿化鞘单胞菌中高度保守。对伊普分解代谢的分子机制的阐明将有助于我们对伊普微生物矿化的理解，并为保守的IPU分解代谢途径的进化提供新的见解。
The worldwide use of the phenylurea herbicide, isoproturon (IPU), has resulted in considerable concern about its environmental fate. Although many microbial metabolites of IPU are known and IPU-mineralizing bacteria have been isolated, the molecular mechanism of IPU catabolism has not been elucidated yet. In this study, complete genes that encode the conserved IPU catabolic pathway were revealed, based on comparative analysis of the genomes of three IPU-mineralizing sphingomonads and subsequent experimental validation. The complete genes included a novel hydrolase gene ddhA, which is responsible for the cleavage of the urea side chain of the IPU demethylated products; a distinct aniline dioxygenase gene cluster adoQTA1A2BR, which has a broad substrate range; and an inducible catechol meta-cleavage pathway gene cluster adoXEGKLIJC. Furthermore, the initial mono-N-demethylation genes pdmAB were further confirmed to be involved in the successive N-demethylation of the IPU mono-N-demethylated product. These IPU-catabolic genes were organized into four transcription units and distributed on three plasmids. They were flanked by multiple mobile genetic elements and highly conserved among IPU-mineralizing sphingomonads. The elucidation of the molecular mechanism of IPU catabolism will enhance our understanding of the microbial mineralization of IPU and provide insights into the evolutionary scenario of the conserved IPU-catabolic pathway.
登录
查看更多内容
影响因子：
12.3
作者：
Hane JK;Rouxel T;Howlett BJ;Kema GH;Goodwin SB;Oliver RP
通讯作者：
Oliver RP
影响因子：
4.5
作者：
Y. Mosleh
通讯作者：
Y. Mosleh
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
4.5
作者：
M. S. da Rocha;L. Arnold;Puttappa R. Dodmane;K. Pennington;Fang Qiu;J. D. de Camargo;S. Cohen
通讯作者：
M. S. da Rocha;L. Arnold;Puttappa R. Dodmane;K. Pennington;Fang Qiu;J. D. de Camargo;S. Cohen
影响因子：
1.7
作者：
P. Gaillardon;M. Sabar
通讯作者：
P. Gaillardon;M. Sabar