Isolation of an MH2 retrovirus mutant temperature sensitive for macrophage but not fibroblast transformation
Isolation of an MH2 retrovirus mutant temperature sensitive for macrophage but not fibroblast transformation
复制标题
分离对巨噬细胞温度敏感但对成纤维细胞转化不敏感的 MH2 逆转录病毒突变体
DOI：
10.1128/jvi.58.1.134-141.1986
复制
发表时间：
1986
影响因子：
5.4
通讯作者：
S. Palmieri
中科院分区：
文献类型：
--
作者：
S. Palmieri
分离到禽MH2逆转录病毒条件突变株ts41MH2。与wtMH2不同，ts41MH2允许转化的巨噬细胞在从37℃到42℃的5至7天的温度变化中分化。突变感染的细胞在42℃孵育后呈现扁平的形态，然后融合形成巨大的多核细胞，非常类似于正常巨噬细胞的体外成熟。这些分化的细胞与髓系巨噬细胞特异性的单抗反应强烈。在温移前将ts41MH2转化的非产毒克隆与含有myc基因的MC29或OK10病毒重叠感染，可抑制分化过程。相反，在重叠感染只含有mIL基因的MH2-PA200病毒的克隆中没有观察到抑制作用。与wtMH2相比，该突变体在静脉注射给雏鸟时，其致癌潜能也有所降低。然而，与造血细胞的结果相反，ts41MH2测试的五个成纤维细胞转化参数中没有一个与wtMH2诱导的参数改变。这些结果表明，ts41MH2的突变位于巨噬细胞转化所必需的myc区域，但不是成纤维细胞转化所必需的。
A conditional mutant of the MH2 avian retrovirus, termed ts41MH2, was isolated. Unlike wtMH2, ts41MH2 permitted transformed macrophages to differentiate during a 5- to 7-day temperature shift from 37 to 42 degrees C. Mutant-infected cells incubated at 42 degrees C exhibited a flattened morphology and then fused to form giant multinucleated cells that closely resembled normal macrophage maturation in vitro. These differentiated cells reacted strongly with a myeloid-macrophage-specific monoclonal antibody. The process of differentiation was inhibited when ts41MH2-transformed nonproducer clones were superinfected before the temperature shift with the myc gene-containing MC29 or OK10 viruses. By contrast, no inhibition was observed in clones superinfected with the MH2-PA200 virus that contains only the mil gene. The mutant also demonstrated a reduced oncogenic potential relative to that of wtMH2 when it was inoculated intravenously into young birds. However, in contrast to the results obtained with hematopoietic cells, none of the five fibroblast transformation parameters tested for ts41MH2 were altered from those induced by wtMH2. These results suggest that the mutation in ts41MH2 is located in a region of myc required for macrophage transformation, but not required for fibroblast transformation.
禽癌病毒MH2的核苷酸序列：两个潜在的onc基因，一个与禽病毒MC29相关，另一个与鼠肉瘤病毒3611相关。
DOI：
10.1073/pnas.81.10.3000
发表时间：
1984
影响因子：
11.1
作者：
Kan,NC;Flordellis,CS;Mark,GE;Duesberg,PH;Papas,TS
通讯作者：
Papas,TS
具有相似转化基因的两种逆转录病毒表现出转化潜力的差异。
影响因子：
3.7
作者：
Linial,M
通讯作者：
Linial,M