Screening differential circular RNA expression profiles reveals hsa_circ_0004018 is associated with hepatocellular carcinoma.
Screening differential circular RNA expression profiles reveals hsa_circ_0004018 is associated with hepatocellular carcinoma.
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DOI：
10.18632/oncotarget.16881
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发表时间：
2017-08-29
期刊：
影响因子：
--
通讯作者：
Guo J
中科院分区：
文献类型：
--
作者：
Fu L;Yao T;Chen Q;Mo X;Hu Y;Guo J
环状RNA （circRNAs）已成为内源性RNA网络不可缺少的组成部分。然而，circRNAs在肝细胞癌（HCC）中的表达意义很少被揭示。本研究的目的是确定circRNA在HCC中的表达谱，并探讨其临床意义和癌症进展的相关机制。通过circRNA微阵列测量HCC中全局circRNA表达谱。通过实时逆转录-聚合酶链反应（real-time逆转录-聚合酶链反应）证实了一个代表性circRNAs hsa_circ_0004018的水平。hsa_circ_0004018在HCC中的表达水平明显低于癌旁组织（P<0.001）。我们的数据进一步表明，hsa_circ_0004018的低表达与血清甲胎蛋白（AFP）水平、肿瘤直径、分化、巴塞罗那临床肝癌分期和肿瘤淋巴结转移分期相关。更重要的是，我们检测了慢性肝炎、肝硬化和HCC患者的肝脏组织；发现hsa_circ_0004018在多种慢性肝病中具有hcc分期特异性表达特征（P<0.001）。受试者工作特征曲线下面积达0.848 （95% CI=0.803 ~ 0.894， P<0.001）。敏感性为0.716，特异性为0.815。最后，hsa_circ_0004018可能通过与mirna的相互作用参与癌症相关途径。
Circular RNAs (circRNAs) have been emerged as an indispensable part of endogenous RNA network. However, the expression significance of circRNAs in hepatocellular carcinoma (HCC) is rarely revealed. The aim of this study was to determine the circRNA expression profile in HCC, and to investigate their clinical significances and relevant mechanisms for cancer progression. The global circRNA expression profile in HCC was measured by circRNA microarray. Levels of one representative circRNAs, hsa_circ_0004018, were confirmed by real-time reverse transcription-polymerase chain reaction. The expression levels of hsa_circ_0004018 in HCC were significantly lower compared with para-tumorous tissue (P<0.001). Our data further showed that lower expression of hsa_circ_0004018 was correlated with serum alpha-fetoprotein (AFP) level, tumor diameters, differentiation, Barcelona Clinic Liver Cancer stage and Tumor-node-metastasis stage. More importantly, we detected liver tissues from chronic hepatitis, cirrhosis and HCC patients; and found that hsa_circ_0004018 harbored HCC-stage-specific expression features in diverse chronic liver diseases (P<0.001). The area under receiver operating characteristic curve was up to 0.848 (95% CI=0.803–0.894, P<0.001). The sensitivity and specificity were 0.716 and 0.815, respectively. Finally, hsa_circ_0004018 might be involved in cancer-related pathways via interactions with miRNAs.
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