Correlation of circular RNA abundance with proliferation--exemplified with colorectal and ovarian cancer, idiopathic lung fibrosis, and normal human tissues.
Correlation of circular RNA abundance with proliferation--exemplified with colorectal and ovarian cancer, idiopathic lung fibrosis, and normal human tissues.
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DOI：
10.1038/srep08057
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发表时间：
2015-01-27
影响因子：
4.6
通讯作者：
Pils D
中科院分区：
文献类型：
--
作者：
Bachmayr-Heyda A;Reiner AT;Auer K;Sukhbaatar N;Aust S;Bachleitner-Hofmann T;Mesteri I;Grunt TW;Zeillinger R;Pils D
环状RNA是最近（重新）发现的丰富的RNA种类，推测其功能为miRNA海绵，因此是竞争性内源RNA网络的一部分。我们分析了匹配的正常结肠粘膜和肿瘤组织中环状和线性RNA的表达和增殖。我们预测了> 1，800个环状RNA，并使用RT-qPCR证明了五个随机选择的例子的存在。有趣的是，与正常结肠样品相比，肿瘤中环状与线性RNA同种型的比率总是较低，在结直肠癌细胞系中甚至更低。此外，该比率与增殖指数呈负相关。在非癌性增殖性疾病、特发性肺纤维化、卵巢癌细胞与培养的正常卵巢上皮细胞和13种正常人体组织中验证了全局环状RNA丰度（circRNA指数）和增殖的相关性。我们是第一个报告结直肠癌细胞系和癌症中环状RNA丰度与正常组织相比总体减少的人，并发现了整体环状RNA丰度与增殖的负相关性。这种负相关性似乎是人体组织中的一般原则，这在三种不同的设置中得到了验证。最后，我们提出了一个简单的模型，环状RNA如何在非增殖细胞中积累。
Circular RNAs are a recently (re-)discovered abundant RNA species with presumed function as miRNA sponges, thus part of the competing endogenous RNA network. We analysed the expression of circular and linear RNAs and proliferation in matched normal colon mucosa and tumour tissues. We predicted >1,800 circular RNAs and proved the existence of five randomly chosen examples using RT-qPCR. Interestingly, the ratio of circular to linear RNA isoforms was always lower in tumour compared to normal colon samples and even lower in colorectal cancer cell lines. Furthermore, this ratio correlated negatively with the proliferation index. The correlation of global circular RNA abundance (the circRNA index) and proliferation was validated in a non-cancerous proliferative disease, idiopathic pulmonary fibrosis, ovarian cancer cells compared to cultured normal ovarian epithelial cells, and 13 normal human tissues. We are the first to report a global reduction of circular RNA abundance in colorectal cancer cell lines and cancer compared to normal tissues and discovered a negative correlation of global circular RNA abundance and proliferation. This negative correlation seems to be a general principle in human tissues as validated with three different settings. Finally, we present a simple model how circular RNAs could accumulate in non-proliferating cells.
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影响因子：
24.5
作者：
Pritchard CC;Grady WM
通讯作者：
Grady WM
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
影响因子：
7
作者：
Castellarin, Mauro;Warren, Rene L.;Holt, Robert A.
通讯作者：
Holt, Robert A.