Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation.
Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation.
复制标题
DOI：
10.1093/nar/gkt296
复制
发表时间：
2013-07
影响因子：
14.9
通讯作者：
Kufel J
中科院分区：
文献类型：
--
作者：
Golisz A;Sikorski PJ;Kruszka K;Kufel J
Sm 样 (Lsm) 蛋白已在所有生物体中被发现，并且与 RNA 代谢相关。在这里，我们报道拟南芥核AtLSM8蛋白以及定位于细胞质和细胞核的AtLSM5在前体mRNA剪接中发挥作用，而AtLSM5和唯一的细胞质AtLSM1则有助于5'–3'mRNA的衰减。在lsm8和sad1/lsm5突变体中，U6小核RNA（snRNA）减少，未剪接的mRNA前体积累，而缺乏AtLSM1和AtLSM5的植物中mRNA稳定性主要受到影响。 lsm1a、lsm1b 和 Sad1/lsm5 植物中受影响的一些 mRNA 也是细胞质 5'–3' 核酸外切酶 AtXRN4 和脱帽酶 AtDCP2 的底物。令人惊讶的是，在缺乏 AtLSM8 的突变体中，一部分底物也稳定下来，这支持了植物 mRNA 在细胞核中主动降解的观点。 LSM 成分的定位、LSM 相互作用蛋白的纯化以及功能分析强烈表明，至少两种在 RNA 代谢中具有保守活性的 LSM 复合物 AtLSM1-7 和 AtLSM2-8 也存在于植物中。
Sm-like (Lsm) proteins have been identified in all organisms and are related to RNA metabolism. Here, we report that Arabidopsis nuclear AtLSM8 protein, as well as AtLSM5, which localizes to both the cytoplasm and nucleus, function in pre-mRNA splicing, while AtLSM5 and the exclusively cytoplasmic AtLSM1 contribute to 5′–3′ mRNA decay. In lsm8 and sad1/lsm5 mutants, U6 small nuclear RNA (snRNA) was reduced and unspliced mRNA precursors accumulated, whereas mRNA stability was mainly affected in plants lacking AtLSM1 and AtLSM5. Some of the mRNAs affected in lsm1a lsm1b and sad1/lsm5 plants were also substrates of the cytoplasmic 5′–3′ exonuclease AtXRN4 and of the decapping enzyme AtDCP2. Surprisingly, a subset of substrates was also stabilized in the mutant lacking AtLSM8, which supports the notion that plant mRNAs are actively degraded in the nucleus. Localization of LSM components, purification of LSM-interacting proteins as well as functional analyses strongly suggest that at least two LSM complexes with conserved activities in RNA metabolism, AtLSM1-7 and AtLSM2-8, exist also in plants.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
DOI：
10.1083/jcb.200910141
发表时间：
2010-04-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Haas G;Braun JE;Igreja C;Tritschler F;Nishihara T;Izaurralde E
通讯作者：
Izaurralde E
影响因子：
4.4
作者：
Anantharaman, V;Aravind, L
通讯作者：
Aravind, L
影响因子：
11.4
作者：
Achsel, T;Brahms, H;Lührmann, R
通讯作者：
Lührmann, R
影响因子：
64.5
作者：
Chekanova, Julia A.;Gregory, Brian D.;Belostotsky, Dmitry A.
通讯作者：
Belostotsky, Dmitry A.