The AMP-Activated Protein Kinase KIN10 Is Involved in the Regulation of Autophagy in Arabidopsis.
The AMP-Activated Protein Kinase KIN10 Is Involved in the Regulation of Autophagy in Arabidopsis.
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AMP 激活的蛋白激酶 KIN10 参与拟南芥自噬的调节
DOI：
10.3389/fpls.2017.01201
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发表时间：
2017
影响因子：
5.6
通讯作者：
Chen QF
中科院分区：
文献类型：
--
作者：
Chen L;Su ZZ;Huang L;Xia FN;Qi H;Xie LJ;Xiao S;Chen QF
自噬是真核生物中高度保守的细胞内成分大量降解和再循环的系统。自噬参与了许多生理过程，包括发育、衰老以及对非生物和生物应激的反应。腺苷5′-单磷酸（AMP）激活的蛋白激酶AMPK正调控哺乳动物的自噬；然而，AMPK在植物自噬中的潜在功能在很大程度上仍然未知。在这里，我们发现了KIN10，哺乳动物AMPK的植物同源物，作为植物自噬的正调节因子，并表明它通过影响拟南芥中ATG1（自噬相关基因1）蛋白的磷酸化起作用。过表达KIN10 （KIN10- oe）的转基因拟南芥株系表现出叶片衰老延迟和对营养饥饿的耐受性增强，这些表型需要功能性自噬途径。与KIN10在自噬中具有潜在作用一致，与野生型相比，营养饥饿诱导的自噬体形成和GFP-ATG8e的裂解在KIN10- oe系中加速。此外，与野生型相比，KIN10-OE系对干旱和缺氧处理的敏感性较低。与野生型相比，碳饥饿提高了KIN10-OE系中磷酸化的YFP-ATG1a的水平。综上所述，这些发现表明，KIN10可能通过影响拟南芥中ATG1s的磷酸化，参与了自噬的正向调节。
Autophagy is a highly conserved system in eukaryotes for the bulk degradation and recycling of intracellular components. Autophagy is involved in many physiological processes including development, senescence, and responses to abiotic and biotic stress. The adenosine 5’-monophosphate (AMP)-activated protein kinase AMPK positively regulates autophagy in mammals; however, the potential function of AMPK in plant autophagy remains largely unknown. Here, we identified KIN10, a plant ortholog of the mammalian AMPK, as a positive regulator of plant autophagy and showed that it acts by affecting the phosphorylation of ATG1 (AUTOPHAGY-RELATED GENE 1) proteins in Arabidopsis. Transgenic Arabidopsis lines overexpressing KIN10 (KIN10-OE) showed delays in leaf senescence, and increased tolerance to nutrient starvation, these phenotypes required a functional autophagy pathway. Consistent with KIN10 having a potential role in autophagy, the nutrient starvation-induced formation of autophagosomes and cleavage of GFP-ATG8e were accelerated in the KIN10-OE lines compared to the wild type. Moreover, the KIN10-OE lines were less sensitive to drought and hypoxia treatments, compared with wild type. Carbon starvation enhanced the level of phosphorylated YFP-ATG1a in the KIN10-OE lines compared to that of wild type. Together, these findings suggest that KIN10 is involved in positive regulation of autophagy, possibly by affecting the phosphorylation of ATG1s in Arabidopsis.
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影响因子：
3.7
作者：
Lee JW;Park S;Takahashi Y;Wang HG
通讯作者：
Wang HG
自噬有助于调节拟南芥淹没过程中的缺氧反应
影响因子：
13.3
作者：
Chen L;Liao B;Qi H;Xie LJ;Huang L;Tan WJ;Zhai N;Yuan LB;Zhou Y;Yu LJ;Chen QF;Shu W;Xiao S
通讯作者：
Xiao S
AMP激活的蛋白激酶对ULK1（HATG1）的磷酸化将能量传感连接到线粒体。
DOI：
10.1126/science.1196371
发表时间：
2011-01-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Egan DF;Shackelford DB;Mihaylova MM;Gelino S;Kohnz RA;Mair W;Vasquez DS;Joshi A;Gwinn DM;Taylor R;Asara JM;Fitzpatrick J;Dillin A;Viollet B;Kundu M;Hansen M;Shaw RJ
通讯作者：
Shaw RJ
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
16
作者：
Kroemer G;Mariño G;Levine B
通讯作者：
Levine B