A pyrosequencing-based metagenomic study of methane-producing microbial community in solid-state biogas reactor.
A pyrosequencing-based metagenomic study of methane-producing microbial community in solid-state biogas reactor.
复制标题
DOI：
10.1186/1754-6834-6-3
复制
发表时间：
2013-01-15
影响因子：
6.3
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Li A;Chu Y;Wang X;Ren L;Yu J;Liu X;Yan J;Zhang L;Wu S;Li S
我国利用各种固体废弃物进行厌氧发酵生产沼气，但其产气效率有待不断提高。相关微生物的多样性和丰富性在生物质产甲烷过程中起着重要作用。下一代高通量焦磷酸测序平台（Roche/454 GS FLX Titanium）为在产芽孢杆菌的微生物群落中发现新微生物提供了强大的工具。为了提高我们的宏基因组分析的能力，我们首先评估了五种不同的方案，用于从产芽孢杆菌的中温固态发酵材料中提取总DNA，然后选择两种高质量的方案进行全面分析。测序读数和组装的重叠群的表征揭示了发酵材料的最普遍的微生物来源于梭菌目（厚壁菌门），其有助于降解蛋白质和纤维素。分解脂肪和碳水化合物的其他重要细菌物种是芽孢杆菌、γ变形菌门和拟杆菌门（分别属于厚壁菌门、变形菌门和拟杆菌门）。优势菌属为梭菌属、氨基杆菌属、嗜冷杆菌属、厌氧球菌属、互养单胞菌属和拟杆菌属。其中，产低温适应性脂肪酶的嗜冷杆菌属和发酵能力较弱的厌氧球菌属是首次在沼气发酵中得到鉴定。甲烷八叠球菌属（Methanosarcina）、甲烷八叠球菌属（Methanosaeta）和甲烷囊菌属（Methanoculleus）是广古菌门（Euryarchaeota）的代表，它们只占整个微生物群落的一小部分。最丰富的古细菌物种包括巴氏甲烷八叠球菌（Methanosarcina barkeri fusaro）、马里黑甲烷菌（Methanoculleus marisnigri JR 1）和嗜热甲烷菌（Methanosaeta theromphila），它们都参与了乙酸营养型和氢营养型甲烷生成。参与沼气生产的新细菌属和种的鉴定为在中温或低温条件下固态发酵的新设计提供了见解。
A solid-state anaerobic digestion method is used to produce biogas from various solid wastes in China but the efficiency of methane production requires constant improvement. The diversity and abundance of relevant microorganisms play important roles in methanogenesis of biomass. The next-generation high-throughput pyrosequencing platform (Roche/454 GS FLX Titanium) provides a powerful tool for the discovery of novel microbes within the biogas-generating microbial communities. To improve the power of our metagenomic analysis, we first evaluated five different protocols for extracting total DNA from biogas-producing mesophilic solid-state fermentation materials and then chose two high-quality protocols for a full-scale analysis. The characterization of both sequencing reads and assembled contigs revealed that the most prevalent microbes of the fermentation materials are derived from Clostridiales (Firmicutes), which contribute to degrading both protein and cellulose. Other important bacterial species for decomposing fat and carbohydrate are Bacilli, Gammaproteobacteria, and Bacteroidetes (belonging to Firmicutes, Proteobacteria, and Bacteroidetes, respectively). The dominant bacterial species are from six genera: Clostridium, Aminobacterium, Psychrobacter, Anaerococcus, Syntrophomonas, and Bacteroides. Among them, abundant Psychrobacter species, which produce low temperature-adaptive lipases, and Anaerococcus species, which have weak fermentation capabilities, were identified for the first time in biogas fermentation. Archaea, represented by genera Methanosarcina, Methanosaeta and Methanoculleus of Euryarchaeota, constitute only a small fraction of the entire microbial community. The most abundant archaeal species include Methanosarcina barkeri fusaro, Methanoculleus marisnigri JR1, and Methanosaeta theromphila, and all are involved in both acetotrophic and hydrogenotrophic methanogenesis. The identification of new bacterial genera and species involved in biogas production provides insights into novel designs of solid-state fermentation under mesophilic or low-temperature conditions.
登录
查看更多内容
影响因子：
4.4
作者：
BEATY, PS;WOFFORD, NQ;MCINERNEY, MJ
通讯作者：
MCINERNEY, MJ
DOI：
10.1007/s11157-008-9131-1
发表时间：
2008-06-01
期刊：
Reviews in Environmental Science and Bio/Technology
影响因子：
--
作者：
Demirel, Burak;Scherer, Paul
通讯作者：
Scherer, Paul
DOI：
10.1099/00207713-33-4-683
发表时间：
1983-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
EZAKI, T;YAMAMOTO, N;YABUUCHI, E
通讯作者：
YABUUCHI, E
影响因子：
4.4
作者：
Hori, T;Haruta, S;Igarashi, Y
通讯作者：
Igarashi, Y
DOI：
10.1099/00207713-49-3-969
发表时间：
1999-07-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Baena, S;Fardeau, ML;Patel, BKC
通讯作者：
Patel, BKC