Azole fungicide sensitivity and molecular mechanisms of reduced sensitivity in Irish Pyrenopeziza brassicae populations.
Azole fungicide sensitivity and molecular mechanisms of reduced sensitivity in Irish Pyrenopeziza brassicae populations.
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爱尔兰 Pyrenopeziza 芸苔种群中唑类杀菌剂敏感性和敏感性降低的分子机制。
DOI：
10.1002/ps.7219
复制
发表时间：
2022
影响因子：
4.1
通讯作者：
Bucur DE
中科院分区：
文献类型：
--
作者：
Bucur DE
背景由甘蓝核盘菌引起的轻斑病是冬季油菜（Brassica napus）的最具破坏性的疾病之一，并且目前甾醇14α-脱甲基酶（CYP 51）抑制剂（唑类）代表用于控制轻斑病发展的主要类别的杀真菌剂。然而，在P。在欧洲不同国家，包括爱尔兰，已经观察到芸苔属的种群。芸苔属植物对唑类杀菌剂的敏感性，3个品种的P.对2018-2020年分离的芸苔属细菌进行了戊唑醇和丙硫菌唑-脱硫的体外抑菌试验，并对该类杀菌剂靶向的PbCYP 51基因在不同菌株中进行了基因分型。唑类杀菌剂的敏感性的变化进行了观察和戊唑醇的敏感性爱尔兰人口之间的差异。在PbCYP 51的启动子区有两个替换（G460 S和S508 T）和不同大小的插入片段。G460 S/S508 T双突变体的存在是首次报道，插入片段大小的多样性大于先前已知的。与野生型菌株相比，携带G460 S或S508 T是不太敏感的杀菌剂，其中插入物也被确定，他们进一步降低敏感性唑类杀菌剂。CONCLUSIONSThe结果这项研究表明，唑类杀菌剂仍然是非常有效的控制轻叶斑病在爱尔兰。然而，建议在具有不同作用模式的杀真菌剂的混合物中使用唑类杀真菌剂。© 2022 The Authors.Pest Management Sciencepublished by John Wiley & Sons Ltd代表化学工业协会。
BACKGROUNDLight leaf spot, caused byPyrenopeziza brassicae, is amongst the most damaging diseases of winter oilseed rape (Brassica napus), and currently the sterol 14α‐demethylase (CYP51) inhibitors (azoles) represent the main class of fungicides used to control light leaf spot development. However, a shift in sensitivity to azole fungicides inP. brassicaepopulations has been observed in different European countries, including Ireland.RESULTSTo assess the sensitivity status of IrishP. brassicaepopulations to azole fungicides, three collections ofP. brassicaefrom 2018–2020 were testedin vitroagainst tebuconazole and prothioconazole‐desthio, and thePbCYP51gene targeted by this class of fungicides was genotyped in different isolates. A change in sensitivity to azole fungicides was observed and differences in sensitivity to tebuconazole between Irish populations were present. There were two substitutions withinPbCYP51(G460S and S508T) and inserts of different sizes in its promoter region. The presence of the G460S/S508T double mutant was reported for the first time, and the diversity in insert size was greater than previously known. Compared to wild type isolates, those carrying G460S or S508T were less sensitive to both fungicides and, where inserts were also identified, they further reduced sensitivity to azole fungicides.CONCLUSIONSThe results of this study suggest that azole fungicides are still very effective in controlling light leaf spot in Ireland. However, using azole fungicides in mixtures of fungicides with different modes of action is recommended. © 2022 The Authors.Pest Management Sciencepublished by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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影响因子：
2.7
作者：
Karolewski, Z.;Fitt, B. D. L.;Evans, N.
通讯作者：
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DOI：
--
发表时间：
2012
期刊：
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作者：
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影响因子：
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作者：
T. Gilles;B. Fitt;R. Kennedy;S. Welham;M. Jeger
通讯作者：
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影响因子：
4.2
作者：
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通讯作者：
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影响因子：
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作者：
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