CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber.
CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber.
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CsMYB60 是黄酮醇和原花青素的关键调节因子，决定黄瓜果刺的颜色
DOI：
10.1093/jxb/ery336
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发表时间：
2019-01-01
影响因子：
6.9
通讯作者：
Ren Z
中科院分区：
文献类型：
--
作者：
Liu M;Zhang C;Duan L;Luan Q;Li J;Yang A;Qi X;Ren Z
Flavonols and proanthocyanidins are the major components that determine the black colouration of cucumber fruit spines and their synthesis is regulated by CsMYB60. Spine colour is an important fruit quality trait that influences the commercial value of cucumber (Cucumis sativus). However, little is known about the metabolites and the regulatory mechanisms of their biosynthesis in black spine varieties. In this study, we determined that the pigments of black spines are flavonoids, including flavonols and proanthocyanidins (PAs). We identified CsMYB60 as the best candidate for the previously identified B (Black spine) locus. Expression levels of CsMYB60 and the key genes involved in flavonoid biosynthesis were higher in black-spine inbred lines than that in white-spine lines at different developmental stages. The insertion of a Mutator-like element (CsMULE) in the second intron of CsMYB60 decreased its expression in a white-spine line. Transient overexpression assays indicated that CsMYB60 is a key regulatory gene and Cs4CL is a key structural gene in the pigmentation of black spines. In addition, the DNA methylation level in the CsMYB60 promoter was much lower in the black-spine line compared with white-spine line. The CsMULE insert may decrease the expression level of CsMYB60, causing hindered synthesis of flavonols and PAs in cucumber fruit spines.
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DOI：
10.1073/pnas.1831119100
发表时间：
2003-09-30
影响因子：
11.1
作者：
May, BP;Liu, H;Martienssen, R
通讯作者：
Martienssen, R
影响因子：
3
作者：
Leo CH;Woodman OL
通讯作者：
Woodman OL
影响因子：
3.1
作者：
Rossi, M;Araujo, PG;Van Sluys, MA
通讯作者：
Van Sluys, MA
影响因子：
3.9
作者：
Ahmad, N;Gupta, S;Mukhtar, H
通讯作者：
Mukhtar, H
一种通过基于准弹性静电力测量振荡周期来绝对确定惯性质量的建议
影响因子：
2.4
作者：
Li, Shisong;Zhang, Zhonghua;Xu, Jinxin
通讯作者：
Xu, Jinxin