Tachyphylaxis to furosemide in isolated airways of guinea pigs.
Tachyphylaxis to furosemide in isolated airways of guinea pigs.
复制标题
豚鼠离体气道对呋塞米的快速耐受。
DOI：
10.1016/0024-3205(96)00416-x
复制
发表时间：
1996
期刊：
影响因子：
6.1
通讯作者：
Nakamura,KT
中科院分区：
文献类型：
--
作者：
Iwamoto,LM;Wilson,VL;Lavallee,SL;Fujiwara,N;Ayau,EL;Nakamura,KT
本体外研究旨在确定豚鼠气道对速尿的快速反应是否发生在对β2-肾上腺素能受体激动剂沙丁胺醇产生快速反应的条件下。用HEPES缓冲液浸泡4-6天新生豚鼠和6周龄雄性豚鼠，测量气管环的等距张力。配对环先用速尿、30或300 μM或对照孵育60分钟，洗涤后用3 μM乙酰胆碱收缩。在稳定收缩时，测量对速尿（30 μM−1 mM）的弛豫。为了进行比较，在10 μM沙丁胺醇孵育30分钟的情况下进行了类似的实验。86rb摄取（K+运输和NaKCl共同运输活性的标志）也在这些气道段中进行了测量。预先接触这些气道松弛剂不影响乙酰胆碱产生的收缩力。观察了沙丁胺醇和呋塞米对气管的脱敏。速尿诱导的松弛在脱敏1小时后部分恢复。预先暴露于300 μM的速尿中并没有抑制正常使用速尿观察到的86rb摄取的减少。综上所述，我们发现：1)在相似的实验条件下，沙丁胺醇和呋塞米对豚鼠气道舒张均发生快速反应；然而2)呋塞米对86rb摄取的抑制作用不受先前暴露的影响。综上所述，这些结果表明，呋塞米诱导的气道松弛可能受到反复或长时间暴露的影响，但这种反应可能与呋塞米敏感NaKCl共转运蛋白活性的变化无关。
This in vitro study was conducted to determine whether tachyphylaxis of guinea pig airway to furosemide occurs under conditions that produce tachyphylaxis to the β2-adrenoceptor agonist, salbutamol. Isometric tension was measured in tracheal rings bathed in HEPES buffer from 4–6 d newborn guinea pigs of either sex, and 6 wk old males. Paired rings were first incubated with furosemide, 30 or 300 μM, or control for 60 min, washed, then constricted with 3 μM acetylcholine. At stable contraction, relaxation to furosemide (30 μM − 1 mM) was measured. For comparison, similar experiments were performed with 10 μM salbutamol incubation for 30 min.86Rb uptake, a marker for K+transport and NaKCl cotransport activity, was also measured in these airway segments. Pre- exposure to these airway relaxants did not affect contractile force generation by acetylcholine. Tracheal desensitization to both salbutamol and furosemide was observed. Partial recovery of furosemide induced relaxation was seen one hour after desensitization. Pre-exposure to 300 μM furosemide did not inhibit the decrease in86Rb uptake normally observed with furosemide. In summary, we found that: 1) tachyphylaxis of guinea pig airway relaxation occurred with both salbutamol and furosemide under similar experimental conditions; however 2) inhibition of86Rb uptake by furosemide was not affected by prior exposure. Taken together, these results suggest that furosemide induced airway relaxation could be affected by repeated or prolonged exposure, but this response may not be associated with changes in furosemide-sensitive NaKCl cotransporter activity.
DOI：
10.3109/17453678308992868
发表时间：
1983-01-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
LINDER, L;ALBREKTSSON, T;LUNDSTROM, I
通讯作者：
LUNDSTROM, I
影响因子：
8.8
作者：
Moon, Hyo-Bang;Choi, Minkyu;Choi, Hee-Gu
通讯作者：
Choi, Hee-Gu