Development and evaluation of microsatellite markers for Acer miyabei (Sapindaceae), a threatened maple species in East Asia.
Development and evaluation of microsatellite markers for Acer miyabei (Sapindaceae), a threatened maple species in East Asia.
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DOI：
10.3732/apps.1500020
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发表时间：
2015-06
影响因子：
3.6
通讯作者：
Kenta T
中科院分区：
文献类型：
--
作者：
Saeki I;Hirao AS;Kenta T
在受威胁的枫树种 Acer miyabei（无患子科）中开发并表征了 12 个微卫星标记，用于群体遗传分析。使用离子个人基因组机 (PGM) 测序，我们开发了具有完美二核苷酸和三核苷酸重复的微卫星标记。这些标记在来自 A. miyabei 亚种的两个自然种群的总共 44 个个体上进行了测试。米亚贝湾miyabei 位于日本北海道岛。每个基因座的等位基因数量从两个到八个不等。每个位点观察到的和预期的杂合度范围分别为 0.05 至 0.75 和 0.05 至 0.79。一些标记已成功转移到密切相关的物种 A. Campestre、A. platanoides 和 A. pictum。开发的标记将有助于表征米亚贝的遗传结构和多样性，并有助于了解其空间遗传变异、近交水平和基因流模式，从而为保护提供基础。
Twelve microsatellite markers were developed and characterized in a threatened maple species, Acer miyabei (Sapindaceae), for use in population genetic analyses. Using Ion Personal Genome Machine (PGM) sequencing, we developed microsatellite markers with perfect di- and trinucleotide repeats. These markers were tested on a total of 44 individuals from two natural populations of A. miyabei subsp. miyabei f. miyabei in Hokkaido Island, Japan. The number of alleles per locus ranged from two to eight. The observed and expected heterozygosities per locus ranged from 0.05 to 0.75 and from 0.05 to 0.79, respectively. Some of the markers were successfully transferred to the closely related species A. campestre, A. platanoides, and A. pictum. The developed markers will be useful in characterizing the genetic structure and diversity of A. miyabei and will help to understand its spatial genetic variation, levels of inbreeding, and patterns of gene flow, thereby providing a basis for conservation.
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