STAT3 promotes tumour progression in glioma by inducing FOXP1 transcription.
STAT3 promotes tumour progression in glioma by inducing FOXP1 transcription.
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STAT3通过诱导FOXP1转录促进神经胶质瘤的肿瘤进展
DOI：
10.1111/jcmm.13837
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发表时间：
2018-11
影响因子：
5.3
通讯作者：
Ke Y
中科院分区：
文献类型：
--
作者：
Sun X;Wang J;Huang M;Chen T;Chen J;Zhang F;Zeng H;Xu Z;Ke Y
本研究探讨STAT 3通过促进FOXP 1的转录对胶质瘤细胞增殖、凋亡和侵袭的影响。应用定量真实的-时间PCR（qRT-PCR）和Western blot分析分别评估胶质瘤组织和细胞中STAT 3和FOXP 1的mRNA和蛋白表达水平。荧光素酶报告基因和染色质免疫沉淀（ChIP）测定，以确定STAT 3和FOXP 1之间的相关性。进行MTT和集落形成测定以鉴定细胞生长。流式细胞仪检测胶质瘤细胞凋亡率。进行Transwell测定以揭示细胞侵袭能力。STAT 3的mRNA和蛋白在胶质瘤组织和细胞中均呈高表达。STAT 3 siRNA转染细胞后，STAT 3和FOXP 1同时下调。STAT 3直接调节FOXP 1的转录。STAT 3通过促进FOXP 1的转录，促进胶质瘤细胞增殖，抑制细胞凋亡，增强细胞侵袭力。综上所述，STAT 3基因是FOXP 1的转录调控因子。STAT 3缺失抑制胶质瘤细胞的增殖和侵袭，促进细胞凋亡。STAT 3和FOXP 1之间的这种分子机制可以作为胶质瘤治疗的治疗靶点。
This paper investigated the effects of STAT3 through promoting FOXP1 transcription on proliferation, apoptosis and invasion in glioma cells. Quantitative real‐time PCR (qRT‐PCR) and Western blot assay were administered to assess the mRNA and protein expression levels of STAT3 and FOXP1 in glioma tissues and cells, respectively. Luciferase reporter and Chromatin Immunoprecipitation (ChIP) assays were implemented to determine the correlation between STAT3 and FOXP1. MTT and colony formation assays were conducted to identify cell growth. Flow cytometry was run to detect the cell apoptosis rate of glioma cells. Transwell assays were conducted to reveal cell invasion ability. The mRNA and protein expression levels of STAT3 were highly expressed in glioma tissues and cells. After cells transfected with siRNA of STAT3, both STAT3 and FOXP1 were simultaneously downregulated. STAT3 directly regulated FOXP1 transcription. STAT3 promoted cell proliferation, inhibited cell apoptosis and enhanced cell invasion through promoting FOXP1 transcription in glioma cells. In summary, STAT3 gene was a transcriptional regulator of FOXP1. Depleted STAT3 restrained cell proliferation and invasion, promoted cell apoptosis in glioma cells. This molecular mechanism between STAT3 and FOXP1 can serve as a therapeutic target for glioma treatment.
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影响因子：
11.2
作者：
Gomez GG;Volinia S;Croce CM;Zanca C;Li M;Emnett R;Gutmann DH;Brennan CW;Furnari FB;Cavenee WK
通讯作者：
Cavenee WK
影响因子：
4.1
作者：
Chang, Nakho;Ahn, Sun Hee;Nam, Do-Hyun
通讯作者：
Nam, Do-Hyun
影响因子：
8
作者：
Amin, HM;McDonnell, TJ;Lai, R
通讯作者：
Lai, R
DOI：
10.1158/1078-0432.ccr-09-1444
发表时间：
2009-09-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Aster J;Kutok J
通讯作者：
Kutok J
影响因子：
12.7
作者：
Claes A;Idema AJ;Wesseling P
通讯作者：
Wesseling P