Mass Spectrometry-Based Sequencing of Lignin Oligomers1[C][W][OA]
Mass Spectrometry-Based Sequencing of Lignin Oligomers1[C][W][OA]
复制标题
基于质谱的木质素低聚物测序1[C][W][OA]
作者：
Kris Morreel;O. Dima;Hoon Kim;F. Lu;Claudiu Niculăeş;Ruben Vanholme;Rebecca Dauwe;G. Goeminne;D. Inzé;E. Messens;J. Ralph;W. Boerjan
虽然蛋白质、核酸和碳水化合物的一级结构可以很容易地确定，但还没有描述地球上第二丰富的生物聚合物(即木质素)的测序方法。在次生增厚的植物细胞壁中，木质素形成芳香的网状结构，源于单醇和许多其他不太丰富的单体的自由基偶联。这种聚合过程导致了过多的单元和连接类型，影响了细胞壁的物理化学特性。目前分析木质素结构的方法只关注主要单体单元和单元间连接类型的频率，而没有提供关于存在不太丰富的未知单元和连接类型的信息，也没有提供连接如何影响相邻连接的形成的信息。这种信息只能使用排序方法来获得。在这里，我们第一次描述了一种利用质谱学对木质素低聚物进行测序的策略。然后对从野生型杨树木质部中提取的低聚物进行了评价。总共观察到134个木素三聚体到六聚体，其中36个可以完全测序。有趣的是，基于未知单体和二聚体亚结构的分子质量数据，观察到至少10个未知单体单元或单元间连接类型，其中一个被鉴定为芳基甘油末端单元。
Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for the second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from the combinatorial radical-radical coupling of monolignols and many other less abundant monomers. This polymerization process leads to a plethora of units and linkage types that affect the physicochemical characteristics of the cell wall. Current methods to analyze the lignin structure focus only on the frequency of the major monomeric units and interunit linkage types but do not provide information on the presence of less abundant unknown units and linkage types, nor on how linkages affect the formation of neighboring linkages. Such information can only be obtained using a sequencing approach. Here, we describe, to our knowledge for the first time, a sequencing strategy for lignin oligomers using mass spectrometry. This strategy was then evaluated on the oligomers extracted from wild-type poplar (Populus tremula × Populus tremuloides) xylem. In total, 134 lignin trimers to hexamers were observed, of which 36 could be completely sequenced. Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit.
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影响因子：
56.9
作者：
Ralph, J;MacKay, JJ;Sederoff, RR
通讯作者：
Sederoff, RR
影响因子：
7.2
作者：
Morreel, Kris;Goeminne, Geert;Boerjan, Wout
通讯作者：
Boerjan, Wout
影响因子：
3.8
作者：
Ralph, J;Lapierre, C;Boerjan, W
通讯作者：
Boerjan, W
DOI：
10.1002/bms.1200160119
发表时间：
1988-10-01
期刊：
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY
影响因子：
--
作者：
BIEMANN, K
通讯作者：
BIEMANN, K