Coacervate delivery of HB-EGF accelerates healing of type 2 diabetic wounds.
Coacervate delivery of HB-EGF accelerates healing of type 2 diabetic wounds.
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DOI：
10.1111/wrr.12319
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Johnson NR;Wang Y
慢性伤口如糖尿病性溃疡是一个重大挑战，因为一些潜在的缺陷阻碍了自然愈合。为了追求再生伤口治疗，我们开发了一种基于肝素的凝聚体递送系统，该系统可以在伤口床内控制释放肝素结合的表皮生长因子（EGF）样生长因子（HB-EGF）。在本研究中，我们使用多基因2型糖尿病小鼠模型来评估HB-EGF凝聚克服糖尿病创面愈合缺陷的能力。在NONcNZO10糖尿病小鼠全层切除伤口中，HB-EGF凝聚体增强表皮角质形成细胞的增殖和迁移，导致上皮化加速。此外，伤口床内胶原沉积的增加导致伤口收缩更快，伤口血管化更大。此外，体外实验表明，从凝聚体中释放的HB-EGF成功地增加了糖尿病人角质形成细胞的迁移。HB-EGF在愈合过程中的多功能作用及其通过凝聚体传递时的增强疗效使其成为治疗糖尿病伤口的一种有希望的方法。
Chronic wounds such as diabetic ulcers pose a significant challenge as a number of underlying deficiencies prevent natural healing. In pursuit of a regenerative wound therapy, we developed a heparin-based coacervate delivery system that provides controlled release of heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) within the wound bed. In this study, we used a polygenic type 2 diabetic mouse model to evaluate the capacity of HB-EGF coacervate to overcome the deficiencies of diabetic wound healing. In full-thickness excisional wounds on NONcNZO10 diabetic mice, HB-EGF coacervate enhanced the proliferation and migration of epidermal keratinocytes, leading to accelerated epithelialization. Furthermore, increased collagen deposition within the wound bed led to faster wound contraction and greater wound vascularization. Additionally, in vitro assays demonstrated that HB-EGF released from the coacervate successfully increased migration of diabetic human keratinocytes. The multifunctional role of HB-EGF in the healing process and its enhanced efficacy when delivered by the coacervate make it a promising therapy for diabetic wounds.
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